Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F20%3A10442516" target="_blank" >RIV/00216208:11310/20:10442516 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61388971:_____/20:00533050 RIV/61388963:_____/20:00533050 RIV/60461373:22310/20:43921852 RIV/60461373:22330/20:43921852
Výsledek na webu
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=S_czcQgXXa" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=S_czcQgXXa</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.carbpol.2020.116588" target="_blank" >10.1016/j.carbpol.2020.116588</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)
Popis výsledku v původním jazyce
Hot water extract from biomass of heterotrophic mutant green alga Parachlorella kessleri HY1 (Chlorellaceae) was deproteinised, and three polysaccharidic fractions were obtained by preparative chromatography. The low-molecular fraction (1.5 x 10(4)g mol(-1)) was defined mainly as branched O-2-beta-xylo-(1 -> 3)-beta-galactofuranan where xylose is partially methylated at O-4. Two high-molecular fractions (3.05 x 10(5) and 9.84 x 10(4)g mol(-1)) were complex polysaccharides containing alpha-L-rhamnan and xylogalactofuranan parts in different ratios. The polysaccharides were well soluble in hot water and, upon cooling, tended to self-segregate. Immunomodulatory activities of the obtained fractions were preliminary tested using ELISA, FACS and ImmunoSpot kits. The polysaccharides increased the TNF-alpha production in melanoma bearing mice with much higher intensity than in healthy mice. This was in agreement with the FACS results on T and B cells indicating their possibly secondary activation by innate immunity cells.
Název v anglickém jazyce
Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)
Popis výsledku anglicky
Hot water extract from biomass of heterotrophic mutant green alga Parachlorella kessleri HY1 (Chlorellaceae) was deproteinised, and three polysaccharidic fractions were obtained by preparative chromatography. The low-molecular fraction (1.5 x 10(4)g mol(-1)) was defined mainly as branched O-2-beta-xylo-(1 -> 3)-beta-galactofuranan where xylose is partially methylated at O-4. Two high-molecular fractions (3.05 x 10(5) and 9.84 x 10(4)g mol(-1)) were complex polysaccharides containing alpha-L-rhamnan and xylogalactofuranan parts in different ratios. The polysaccharides were well soluble in hot water and, upon cooling, tended to self-segregate. Immunomodulatory activities of the obtained fractions were preliminary tested using ELISA, FACS and ImmunoSpot kits. The polysaccharides increased the TNF-alpha production in melanoma bearing mice with much higher intensity than in healthy mice. This was in agreement with the FACS results on T and B cells indicating their possibly secondary activation by innate immunity cells.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10404 - Polymer science
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Carbohydrate Polymers
ISSN
0144-8617
e-ISSN
—
Svazek periodika
246
Číslo periodika v rámci svazku
October
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
11
Strana od-do
116588
Kód UT WoS článku
000554907700012
EID výsledku v databázi Scopus
2-s2.0-85086898107