Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00533050" target="_blank" >RIV/61388963:_____/20:00533050 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/20:00533050 RIV/60461373:22310/20:43921852 RIV/60461373:22330/20:43921852 RIV/00216208:11310/20:10442516

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0144861720307621" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0144861720307621</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.carbpol.2020.116588" target="_blank" >10.1016/j.carbpol.2020.116588</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)

Popis výsledku v původním jazyce

Hot water extract from biomass of heterotrophic mutant green alga Parachlorella kessleri HY1 (Chlorellaceae) was deproteinised, and three polysaccharidic fractions were obtained by preparative chromatography. The low-molecular fraction (1.5 x 10(4)g mol(-1)) was defined mainly as branched O-2-beta-xylo-(1> 3)-beta-galactofuranan where xylose is partially methylated at O-4. Two high-molecular fractions (3.05 x 10(5) and 9.84 x 10(4)g mol(-1)) were complex polysaccharides containing alpha-L-rhamnan and xylogalactofuranan parts in different ratios. The polysaccharides were well soluble in hot water and, upon cooling, tended to self-segregate. Immunomodulatory activities of the obtained fractions were preliminary tested using ELISA, FACS and ImmunoSpot kits. The polysaccharides increased the TNF-alpha production in melanoma bearing mice with much higher intensity than in healthy mice. This was in agreement with the FACS results on T and B cells indicating their possibly secondary activation by innate immunity cells.

Název v anglickém jazyce

Immunoactive polysaccharides produced by heterotrophic mutant of green microalga Parachlorella kessleri HY1 (Chlorellaceae)

Popis výsledku anglicky

Hot water extract from biomass of heterotrophic mutant green alga Parachlorella kessleri HY1 (Chlorellaceae) was deproteinised, and three polysaccharidic fractions were obtained by preparative chromatography. The low-molecular fraction (1.5 x 10(4)g mol(-1)) was defined mainly as branched O-2-beta-xylo-(1> 3)-beta-galactofuranan where xylose is partially methylated at O-4. Two high-molecular fractions (3.05 x 10(5) and 9.84 x 10(4)g mol(-1)) were complex polysaccharides containing alpha-L-rhamnan and xylogalactofuranan parts in different ratios. The polysaccharides were well soluble in hot water and, upon cooling, tended to self-segregate. Immunomodulatory activities of the obtained fractions were preliminary tested using ELISA, FACS and ImmunoSpot kits. The polysaccharides increased the TNF-alpha production in melanoma bearing mice with much higher intensity than in healthy mice. This was in agreement with the FACS results on T and B cells indicating their possibly secondary activation by innate immunity cells.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Carbohydrate Polymers

ISSN

0144-8617

e-ISSN

—

Svazek periodika

246

Číslo periodika v rámci svazku

OCT 15

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

116588

Kód UT WoS článku

000554907700012

EID výsledku v databázi Scopus

2-s2.0-85086898107