Production of Virus-Free Garlic Plants through Somatic Embryogenesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F21%3A10432700" target="_blank" >RIV/00216208:11310/21:10432700 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=5By_SSfUzG" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=5By_SSfUzG</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/agronomy11050876" target="_blank" >10.3390/agronomy11050876</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Production of Virus-Free Garlic Plants through Somatic Embryogenesis

Popis výsledku v původním jazyce

The present study was conducted to establish a protocol for the regeneration of virus-free garlic plants through somatic embryogenesis of two Croatian garlic ecotypes. Basal parts of cloves from mother plants were cultured on a full Murashige and Skoog (MS) or modified MS medium (1/4 of KNO3 and NH4NO3 and 2xMgSO(4)) containing 0.1 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) or 1 mg L-1 2,4-D + 0.5 mg L-1 kinetin (Kin) and representing four different treatments. Plants were regenerated in MS medium containing 0.1 mg L-1 2,4-D and rooted in a medium containing 0.05 mg L-1 1-naphthaleneacetic acid (NAA) + 0.005 mg L-1 6-(gamma,gamma-dimethylallylamino)purine (2iP). The presence of viruses (i.e., sanitary status) of the mother plants and regenerants was checked by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). The mother plants were infected with onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV). In addition, the presence of garlic common latent virus (GCLV) was confirmed in four mother plants. Embryogenic callus developed in all four treatments with success ranging from 55% to 81% depending on treatment and ecotype. Plant conversion was significantly higher in somatic embryos developed in media containing 0.1 mg L-1 2,4-D than those developed in media containing 1 mg L-1 2,4-D + 0.5 mg L-1 Kin. Virus elimination success ranged from 13.3% up to 62.5% depending on garlic ecotype and treatment. The overall rate of virus elimination by somatic embryogenesis for both treatments and ecotypes were 20.7%, 22.9%, and 30.5% for OYDV, GCLV, and LYSV, respectively. Based on these results, somatic embryogenesis has been shown to be equally or more successful in eliminating garlic viruses compared to other in vitro methods.

Název v anglickém jazyce

Production of Virus-Free Garlic Plants through Somatic Embryogenesis

Popis výsledku anglicky

The present study was conducted to establish a protocol for the regeneration of virus-free garlic plants through somatic embryogenesis of two Croatian garlic ecotypes. Basal parts of cloves from mother plants were cultured on a full Murashige and Skoog (MS) or modified MS medium (1/4 of KNO3 and NH4NO3 and 2xMgSO(4)) containing 0.1 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D) or 1 mg L-1 2,4-D + 0.5 mg L-1 kinetin (Kin) and representing four different treatments. Plants were regenerated in MS medium containing 0.1 mg L-1 2,4-D and rooted in a medium containing 0.05 mg L-1 1-naphthaleneacetic acid (NAA) + 0.005 mg L-1 6-(gamma,gamma-dimethylallylamino)purine (2iP). The presence of viruses (i.e., sanitary status) of the mother plants and regenerants was checked by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). The mother plants were infected with onion yellow dwarf virus (OYDV) and leek yellow stripe virus (LYSV). In addition, the presence of garlic common latent virus (GCLV) was confirmed in four mother plants. Embryogenic callus developed in all four treatments with success ranging from 55% to 81% depending on treatment and ecotype. Plant conversion was significantly higher in somatic embryos developed in media containing 0.1 mg L-1 2,4-D than those developed in media containing 1 mg L-1 2,4-D + 0.5 mg L-1 Kin. Virus elimination success ranged from 13.3% up to 62.5% depending on garlic ecotype and treatment. The overall rate of virus elimination by somatic embryogenesis for both treatments and ecotypes were 20.7%, 22.9%, and 30.5% for OYDV, GCLV, and LYSV, respectively. Based on these results, somatic embryogenesis has been shown to be equally or more successful in eliminating garlic viruses compared to other in vitro methods.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Agronomy [online]

ISSN

2073-4395

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

876

Kód UT WoS článku

000653305200001

EID výsledku v databázi Scopus

2-s2.0-85106478468