MHC II-EGFP knock-in mouse model is a suitable tool for systems and quantitative immunology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F22%3A10451214" target="_blank" >RIV/00216208:11310/22:10451214 - isvavai.cz</a>

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=m8RzybQew" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=m8RzybQew</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.imlet.2022.10.007" target="_blank" >10.1016/j.imlet.2022.10.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

MHC II-EGFP knock-in mouse model is a suitable tool for systems and quantitative immunology

Popis výsledku v původním jazyce

Immunology is a rapidly evolving field of research with sophisticated models and methods. However, detailed data on total immune cell counts and population distributions remain surprisingly scarce. Nevertheless, recently established quantitative approaches could help us understand the overall complexity of the immune system. Here, we studied a major histocompatibility complexclass II - enhanced green fluorescent protein knock-in mouse model to precisely identify and manipulate lymphoid structures. By combining flow cytometry with light sheet microscopy, we quantified MHC II+ populations of the small intestine and associated individual mesenteric lymph nodes, with 36.7 x 10(6) cells in lamina propria, 3.0 x 10(5) cells in scattered lymphoid tissue and 1.1 x 10(6) cells in Peyer&apos;s patches. In addition to these whole-organ cell counts, we assessed approximately 1 x 10(6) total villi in the small intestine and 450 scattered lymphoid tissue follicles. By direct noninvasive microscopic observation of a naturally fully translucent mouse organ, the cornea, we quantified 12 +/- 4 and 35 +/- 7 cells/mm(2) Langerhans-and macrophage-like populations, respectively. Ultimately, our findings show that flow cytometry with quantitative imaging data analysis enables us to avoid methodological discrepancies while gaining new insights into the relevance of organ-specific quantitative approaches for immunology.

Název v anglickém jazyce

MHC II-EGFP knock-in mouse model is a suitable tool for systems and quantitative immunology

Popis výsledku anglicky

Immunology is a rapidly evolving field of research with sophisticated models and methods. However, detailed data on total immune cell counts and population distributions remain surprisingly scarce. Nevertheless, recently established quantitative approaches could help us understand the overall complexity of the immune system. Here, we studied a major histocompatibility complexclass II - enhanced green fluorescent protein knock-in mouse model to precisely identify and manipulate lymphoid structures. By combining flow cytometry with light sheet microscopy, we quantified MHC II+ populations of the small intestine and associated individual mesenteric lymph nodes, with 36.7 x 10(6) cells in lamina propria, 3.0 x 10(5) cells in scattered lymphoid tissue and 1.1 x 10(6) cells in Peyer&apos;s patches. In addition to these whole-organ cell counts, we assessed approximately 1 x 10(6) total villi in the small intestine and 450 scattered lymphoid tissue follicles. By direct noninvasive microscopic observation of a naturally fully translucent mouse organ, the cornea, we quantified 12 +/- 4 and 35 +/- 7 cells/mm(2) Langerhans-and macrophage-like populations, respectively. Ultimately, our findings show that flow cytometry with quantitative imaging data analysis enables us to avoid methodological discrepancies while gaining new insights into the relevance of organ-specific quantitative approaches for immunology.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30102 - Immunology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Immunology Letters

ISSN

0165-2478

e-ISSN

1879-0542

Svazek periodika

251-252

Číslo periodika v rámci svazku

12/2022

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

75-85

Kód UT WoS článku

000886916300003

EID výsledku v databázi Scopus

2-s2.0-85141831116