Fish parvalbumin gene: Detection and quantification by universal primers for forensic application

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F24%3A10481057" target="_blank" >RIV/00216208:11310/24:10481057 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027022:_____/24:N0000034 RIV/60461373:22330/24:43928371

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=SdTubG~KGB" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=SdTubG~KGB</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jfca.2024.106029" target="_blank" >10.1016/j.jfca.2024.106029</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fish parvalbumin gene: Detection and quantification by universal primers for forensic application

Popis výsledku v původním jazyce

The substitution of illegal fish species poses economic, health and environmental risks. Identifying fish species, particularly closely related ones lacking external features, is a challenge. This study introduces an alternative approach using nuclear gene markers for positive detection, identification, and quantification of mitochondrial gene markers common in existing assays. We developed a universal primer targeting the parvalbumin (pvalb) beta gene, a common fish allergen with an amplicon size of 117 bp long. The developed fish-specific primer assay was tested on a diverse panel of 54 fish species, including commonly consumed species such as salmon, carp, and cod, along with non-fish species such as Buffalo, chicken and pork, and vegetables such as wheat, celery, and mint. The assay consistently identified the fish pvalb gene, demonstrating our assay&apos;s high specificity and effectiveness in detecting this gene in a variety of fish species. The assay exhibited a low limit of detection (LOD) of 5 pg, detecting trace DNA quantities. The assay also effectively quantified parvalbumin with good efficiency and linearity. These findings highlight pvalb as a reliable forensic tool for the identification of fish species. Implementing nuclear gene markers mitigates fish mislabelling and fraud, reducing economic and environmental impacts.

Název v anglickém jazyce

Fish parvalbumin gene: Detection and quantification by universal primers for forensic application

Popis výsledku anglicky

The substitution of illegal fish species poses economic, health and environmental risks. Identifying fish species, particularly closely related ones lacking external features, is a challenge. This study introduces an alternative approach using nuclear gene markers for positive detection, identification, and quantification of mitochondrial gene markers common in existing assays. We developed a universal primer targeting the parvalbumin (pvalb) beta gene, a common fish allergen with an amplicon size of 117 bp long. The developed fish-specific primer assay was tested on a diverse panel of 54 fish species, including commonly consumed species such as salmon, carp, and cod, along with non-fish species such as Buffalo, chicken and pork, and vegetables such as wheat, celery, and mint. The assay consistently identified the fish pvalb gene, demonstrating our assay&apos;s high specificity and effectiveness in detecting this gene in a variety of fish species. The assay exhibited a low limit of detection (LOD) of 5 pg, detecting trace DNA quantities. The assay also effectively quantified parvalbumin with good efficiency and linearity. These findings highlight pvalb as a reliable forensic tool for the identification of fish species. Implementing nuclear gene markers mitigates fish mislabelling and fraud, reducing economic and environmental impacts.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10511 - Environmental sciences (social aspects to be 5.7)

Projekt

<a href="/cs/project/QK1910231" target="_blank" >QK1910231: Nové přístupy k průkazu falšování rybího masa pomocí genomové DNA</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food Composition and Analysis

ISSN

0889-1575

e-ISSN

1096-0481

Svazek periodika

128

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

106029

Kód UT WoS článku

001187947800001

EID výsledku v databázi Scopus

2-s2.0-85185474560