Drop coating deposition Raman (DCDR) spectroscopy of liposomes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F11%3A10081033" target="_blank" >RIV/00216208:11320/11:10081033 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1002/jrs.2915" target="_blank" >http://dx.doi.org/10.1002/jrs.2915</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jrs.2915" target="_blank" >10.1002/jrs.2915</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Drop coating deposition Raman (DCDR) spectroscopy of liposomes
Popis výsledku v původním jazyce
Drop coating deposition Raman (DCDR) spectroscopy was tested as a potential technique for studying liposomes at very low sample concentrations. We used model liposomes prepared either from 1,2-distearoyl-sn-glycero-3-phospocholine (PC) or from soybean asolectin that is composed of various lipids and thus represents a good model of natural membranes. In both cases, deposited samples formed a dried drop with round shape and a ring of concentrated liposomes at the edge. Spectral mapping showed that maximalRaman intensity originated from the inner part of the edge ring, while Raman signal gradually decreased in both radial directions. Raman spectra exhibited excellent reproducibility of spectral characteristics at different locations in the drop indicating similar conformation and ordering of hydrocarbon lipid chains in the sample. Our results suggest that DCDR spectroscopy can be used for studying lipids in situ, while sensitivity of this technique is at least 2 orders of magnitude highe
Název v anglickém jazyce
Drop coating deposition Raman (DCDR) spectroscopy of liposomes
Popis výsledku anglicky
Drop coating deposition Raman (DCDR) spectroscopy was tested as a potential technique for studying liposomes at very low sample concentrations. We used model liposomes prepared either from 1,2-distearoyl-sn-glycero-3-phospocholine (PC) or from soybean asolectin that is composed of various lipids and thus represents a good model of natural membranes. In both cases, deposited samples formed a dried drop with round shape and a ring of concentrated liposomes at the edge. Spectral mapping showed that maximalRaman intensity originated from the inner part of the edge ring, while Raman signal gradually decreased in both radial directions. Raman spectra exhibited excellent reproducibility of spectral characteristics at different locations in the drop indicating similar conformation and ordering of hydrocarbon lipid chains in the sample. Our results suggest that DCDR spectroscopy can be used for studying lipids in situ, while sensitivity of this technique is at least 2 orders of magnitude highe
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
BO - Biofyzika
OECD FORD obor
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Návaznosti výsledku
Projekt
—
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Raman Spectroscopy
ISSN
0377-0486
e-ISSN
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Svazek periodika
42
Číslo periodika v rámci svazku
8
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
5
Strana od-do
1606-1610
Kód UT WoS článku
000294767700002
EID výsledku v databázi Scopus
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