Biohybrid architectures for efficient light-to-current conversion based on photosystem I within scalable 3D mesoporous electrodes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11320%2F16%3A10331008" target="_blank" >RIV/00216208:11320/16:10331008 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1039/c6ta07141d" target="_blank" >http://dx.doi.org/10.1039/c6ta07141d</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c6ta07141d" target="_blank" >10.1039/c6ta07141d</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biohybrid architectures for efficient light-to-current conversion based on photosystem I within scalable 3D mesoporous electrodes

Popis výsledku v původním jazyce

The combination of advanced materials and defined surface design with complex proteins from natural photosynthesis is currently one of the major topics in the development of biohybrid systems and biophotovoltaic devices. In this study transparent mesoporous indium tin oxide (mu ITO) electrodes have been used in combination with the trimeric supercomplex photosystem I (PSI) from Thermosynechococcus elongatus and the small redox protein cytochrome c (cyt c) from horse heart to fabricate advanced and efficient photobiocathodes. The preparation of the mu ITO via spin coating allows easy scalability and ensures a defined increase in the electrochemically active surface area with accessibility for both proteins. Using these 3D electrodes up to 40 mm thickness, the immobilization of cyt c and PSI with full monolayer coverage and their electrical communication to the electrode can be achieved. Significant improvement can be made when the heterogenous electron transfer rate constant of cyt c with the electrode is increased by an appropriate surface treatment. The photocurrent follows linearly the thickness of the mu ITO and current densities of up to 150 mA cm(-2) can be obtained without indications of a limitation. The internal quantum efficiency is determined to be 39% which demonstrates that the wiring of PSI via cyt c can be advantageously used in a system with high protein loading and efficient electron pathways inside 3D transparent conducting oxides.

Název v anglickém jazyce

Biohybrid architectures for efficient light-to-current conversion based on photosystem I within scalable 3D mesoporous electrodes

Popis výsledku anglicky

The combination of advanced materials and defined surface design with complex proteins from natural photosynthesis is currently one of the major topics in the development of biohybrid systems and biophotovoltaic devices. In this study transparent mesoporous indium tin oxide (mu ITO) electrodes have been used in combination with the trimeric supercomplex photosystem I (PSI) from Thermosynechococcus elongatus and the small redox protein cytochrome c (cyt c) from horse heart to fabricate advanced and efficient photobiocathodes. The preparation of the mu ITO via spin coating allows easy scalability and ensures a defined increase in the electrochemically active surface area with accessibility for both proteins. Using these 3D electrodes up to 40 mm thickness, the immobilization of cyt c and PSI with full monolayer coverage and their electrical communication to the electrode can be achieved. Significant improvement can be made when the heterogenous electron transfer rate constant of cyt c with the electrode is increased by an appropriate surface treatment. The photocurrent follows linearly the thickness of the mu ITO and current densities of up to 150 mA cm(-2) can be obtained without indications of a limitation. The internal quantum efficiency is determined to be 39% which demonstrates that the wiring of PSI via cyt c can be advantageously used in a system with high protein loading and efficient electron pathways inside 3D transparent conducting oxides.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

—

Projekt

<a href="/cs/project/GBP501%2F12%2FG055" target="_blank" >GBP501/12/G055: Centrum fotosyntetického výzkumu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Materials Chemistry A

ISSN

2050-7488

e-ISSN

—

Svazek periodika

4

Číslo periodika v rámci svazku

43

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

17009-17017

Kód UT WoS článku

000387878700031

EID výsledku v databázi Scopus

2-s2.0-84994226193