POSSIBILITY OF USE OF DISC DIFFUSION METHOD IN INQUIRY-BASED TASKS

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

POSSIBILITY OF USE OF DISC DIFFUSION METHOD IN INQUIRY-BASED TASKS

Popis výsledku v původním jazyce

Disc diffusion method is a basic method for testing susceptibility of bacteria to antibiotics. The principle consists in placing antibiotic discs onto agar plates that have already been inoculated with selected sample of bacteria. The plates are then incubated overnight. After incubation, the zones of inhibition surrounding the antibiotic discs can be seen. In school conditions the nonpathogenic bacteria isolated from environment can be used very well for this test. If teacher chooses antibiotics with different mechanism of effect, students can after the test easily find out that each antibiotic has variously strong effect to one species/strain of bacteria. This effect depends on metabolism and composition of bacteria. In the same manner the susceptibility of other microorganisms, e.g. yeast or mould, can be tested. In this case, students can see no inhibition zones on the agar plate and can find out that antibiotics have no effect on yeast or mould. Experiments described in this paper can be used in inquiry-based education tasks.

Název v anglickém jazyce

POSSIBILITY OF USE OF DISC DIFFUSION METHOD IN INQUIRY-BASED TASKS

Popis výsledku anglicky

Disc diffusion method is a basic method for testing susceptibility of bacteria to antibiotics. The principle consists in placing antibiotic discs onto agar plates that have already been inoculated with selected sample of bacteria. The plates are then incubated overnight. After incubation, the zones of inhibition surrounding the antibiotic discs can be seen. In school conditions the nonpathogenic bacteria isolated from environment can be used very well for this test. If teacher chooses antibiotics with different mechanism of effect, students can after the test easily find out that each antibiotic has variously strong effect to one species/strain of bacteria. This effect depends on metabolism and composition of bacteria. In the same manner the susceptibility of other microorganisms, e.g. yeast or mould, can be tested. In this case, students can see no inhibition zones on the agar plate and can find out that antibiotics have no effect on yeast or mould. Experiments described in this paper can be used in inquiry-based education tasks.

Druh

D - Stať ve sborníku

CEP obor

AM - Pedagogika a školství

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

PROJEKTOVE VYUCOVANI V PRIRODNICH PREDMETECH

ISBN

978-80-7290-864-6

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

102-107

Název nakladatele

Univerzita Karlova v Praze, Pedagogická fakulta

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

29. 10. 2015

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000375780600015