The dysregulation of monocyte subpopulations in individuals at risk of developing rheumatoid arthritis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11510%2F21%3A10428540" target="_blank" >RIV/00216208:11510/21:10428540 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023728:_____/21:N0000074 RIV/00216208:11110/21:10428540

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=GhIwFWUekE" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=GhIwFWUekE</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/rheumatology/keaa518" target="_blank" >10.1093/rheumatology/keaa518</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The dysregulation of monocyte subpopulations in individuals at risk of developing rheumatoid arthritis

Popis výsledku v původním jazyce

Objectives. Individuals carrying antibodies against citrullinated proteins (ACPA) are at high risk of developing RA. EULAR provided a clinical definition of individuals with arthralgia suspicious for progression to RA (clinically suspect arthralgia, CSA). The alteration of monocyte subpopulations in patients with established RA has been previously described. We analysed peripheral blood monocyte subpopulations in individuals with arthralgia at risk of RA. Methods. We included 70 at-risk individuals, defined as having arthralgia without arthritis and being either ACPA(+) or meeting the clinical CSA definition, 23 patients with early RA (ERA) and 19 healthy controls (HCs). Monocytes classified as classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+/++)) and nonclassical (CD14(-/+)CD16(++)) were analysed by flow cytometry. Results. Of the 70 at-risk individuals, 46 were ACPA(+) and 45 met the CSA definition. The at-risk individuals and, especially, ERA patients had a lower percentage of classical monocytes and a higher percentage of nonclassical monocytes than the HCs. ACPA positivity had no effect on the difference in the distribution of the monocyte subsets between at-risk individuals and ERA patients, but a difference was determined in those reaching the ERA phase. However, when compared with HCs, the shift of monocyte subsets was more significant in ACPA(+) than in ACPA(-) individuals with arthralgia. This trend was observed in individuals who did not meet the CSA definition. This finding was, however, determined by a selection bias, as these individuals were solely ACPA(+). Conclusion. The shift from classical to nonclassical monocyte subpopulations was observed already in individuals at risk of developing RA.

Název v anglickém jazyce

The dysregulation of monocyte subpopulations in individuals at risk of developing rheumatoid arthritis

Popis výsledku anglicky

Objectives. Individuals carrying antibodies against citrullinated proteins (ACPA) are at high risk of developing RA. EULAR provided a clinical definition of individuals with arthralgia suspicious for progression to RA (clinically suspect arthralgia, CSA). The alteration of monocyte subpopulations in patients with established RA has been previously described. We analysed peripheral blood monocyte subpopulations in individuals with arthralgia at risk of RA. Methods. We included 70 at-risk individuals, defined as having arthralgia without arthritis and being either ACPA(+) or meeting the clinical CSA definition, 23 patients with early RA (ERA) and 19 healthy controls (HCs). Monocytes classified as classical (CD14(++)CD16(-)), intermediate (CD14(++)CD16(+/++)) and nonclassical (CD14(-/+)CD16(++)) were analysed by flow cytometry. Results. Of the 70 at-risk individuals, 46 were ACPA(+) and 45 met the CSA definition. The at-risk individuals and, especially, ERA patients had a lower percentage of classical monocytes and a higher percentage of nonclassical monocytes than the HCs. ACPA positivity had no effect on the difference in the distribution of the monocyte subsets between at-risk individuals and ERA patients, but a difference was determined in those reaching the ERA phase. However, when compared with HCs, the shift of monocyte subsets was more significant in ACPA(+) than in ACPA(-) individuals with arthralgia. This trend was observed in individuals who did not meet the CSA definition. This finding was, however, determined by a selection bias, as these individuals were solely ACPA(+). Conclusion. The shift from classical to nonclassical monocyte subpopulations was observed already in individuals at risk of developing RA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30226 - Rheumatology

Projekt

<a href="/cs/project/NV17-32612A" target="_blank" >NV17-32612A: Klinicky suspektní artralgie: charakteristika preklinické fáze revmatoidní artritidy</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Rheumatology

ISSN

1462-0324

e-ISSN

—

Svazek periodika

60

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

1823-1831

Kód UT WoS článku

000644544400044

EID výsledku v databázi Scopus

2-s2.0-85104048166