Transcriptom Treponema pallidum: expresní profilování během experimentální infekce králíků

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F03%3A00012310" target="_blank" >RIV/00216224:14110/03:00012310 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection

Popis výsledku v původním jazyce

DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) during infection of rabbits. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum(Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. Total RNA from T. pallidum isolated from rabbit testes 10 days post infection was labeled and standardized by cohybridization of the same arrays with treponemal chromosomal DNA labeled with a different fluorescent marker. This internal standardization technique proved to be highly reproducible and to decrease the impact of variables such as host nucleic acid contamination or variable target DNA lengths. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicati

Název v anglickém jazyce

Transcriptome of Treponema pallidum: gene expression profile during experimental rabbit infection

Popis výsledku anglicky

DNA microarray technology was utilized to study gene expression by the syphilis spirochete Treponema pallidum subsp. pallidum (Nichols) during infection of rabbits. Microarrays containing all 1039 annotated ORFs of Treponema pallidum subspecies pallidum(Nichols) were printed on glass slides. For 1034 ORFs (out of 1039), signals higher than the threshold (average of negative control spots + 3 SDs) were detected for both RNA and DNA probes. Total RNA from T. pallidum isolated from rabbit testes 10 days post infection was labeled and standardized by cohybridization of the same arrays with treponemal chromosomal DNA labeled with a different fluorescent marker. This internal standardization technique proved to be highly reproducible and to decrease the impact of variables such as host nucleic acid contamination or variable target DNA lengths. The most highly transcribed genes were found to correlate with the most conspicuous spots identified by two dimensional gel electrophoresis, indicati

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/NI7351" target="_blank" >NI7351: Komparativní genomika patogenních spirochet rodu Treponema: cesta k sekvenčně specifické diagnostice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

CHEMICKÉ LISTY

ISSN

0009-2770

e-ISSN

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Svazek periodika

2003

Číslo periodika v rámci svazku

97

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

1

Strana od-do

311

Kód UT WoS článku

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EID výsledku v databázi Scopus

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