Interakcia haloperidolu s L-typom vápníkového kanála

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F05%3A00013059" target="_blank" >RIV/00216224:14110/05:00013059 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of haloperidol with L-type voltage dependent calcium channels

Popis výsledku v původním jazyce

Haloperidol is neuroleptic agent, effective in the treatment of various psychiatric disorders, such as schizophrenia or mania. Cardiac arrhythmias and other severe cardiovascular side effects have been associated with this therapy. We have investigated effects of haloperidol on currents through cardiac calcium channels in native tissue, i.e., in cardiomyocytes, and in expression system, i.e., in HEK 293 cells. Cardiomyocytes were enzymaticaly isolated from adult rat ventricles. HEK 293 cells were transiently transfected with cDNAs encoding a and b isoforms of the a1 subunit of CaV1.2 calcium channel together with b2a and a2d subunits. a1C-a isoform is expressed mostly in cardiac tissue, while a1C-b is prevalent in smooth muscles. Currents were measuredusing whole cell configuration of patch-clamp method. In HEK 293 cells transfected with a1C-b subunit 10 ľmol/l haloperidol inhibited approximately 58% of inward current amplitude measured in peak of IV relation. The inhibition was rever

Název v anglickém jazyce

Interaction of haloperidol with L-type voltage dependent calcium channels

Popis výsledku anglicky

Haloperidol is neuroleptic agent, effective in the treatment of various psychiatric disorders, such as schizophrenia or mania. Cardiac arrhythmias and other severe cardiovascular side effects have been associated with this therapy. We have investigated effects of haloperidol on currents through cardiac calcium channels in native tissue, i.e., in cardiomyocytes, and in expression system, i.e., in HEK 293 cells. Cardiomyocytes were enzymaticaly isolated from adult rat ventricles. HEK 293 cells were transiently transfected with cDNAs encoding a and b isoforms of the a1 subunit of CaV1.2 calcium channel together with b2a and a2d subunits. a1C-a isoform is expressed mostly in cardiac tissue, while a1C-b is prevalent in smooth muscles. Currents were measuredusing whole cell configuration of patch-clamp method. In HEK 293 cells transfected with a1C-b subunit 10 ľmol/l haloperidol inhibited approximately 58% of inward current amplitude measured in peak of IV relation. The inhibition was rever

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA305%2F04%2F1385" target="_blank" >GA305/04/1385: Modulační úloha sigma signalizace na elektromechanické vztahy izolovaného kardiomyocytu a srdce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

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Svazek periodika

54

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

1

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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