Yeast and fungal cell-wall polysaccharides can self-assemble in vitro into an ultrastructure resembling in vivo yeast cell walls

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00067534" target="_blank" >RIV/00216224:14110/13:00067534 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1093/jmicro/dfs076" target="_blank" >http://dx.doi.org/10.1093/jmicro/dfs076</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/jmicro/dfs076" target="_blank" >10.1093/jmicro/dfs076</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Yeast and fungal cell-wall polysaccharides can self-assemble in vitro into an ultrastructure resembling in vivo yeast cell walls

Popis výsledku v původním jazyce

Polysaccharides account for more than 90% of the content of fungal cell walls, but the mechanism underlying the formation of the architecture of the cell walls, which consist of microfibrils embedded in an amorphous wall matrix, remains unknown. We usedelectron microscopy to investigate ten different fungal cell-wall polysaccharides to determine whether they could self-assemble into the fibrillar or amorphous component of fungal cell walls in a test tube without enzymes. The ultrastructures formed by precipitating beta-1,3-glucan and beta-1,6-glucan are different depending on the existence of branching in the molecule. Linear beta-1,3- glucan and linear beta-1,6-glucan precipitate into a fibrillar ultrastructure. Branched beta-1,6-glucan, mannan and glycogen precipitates are amorphous. Branched beta-1,3-glucan forms a fibrillar plus amorphous ultrastructure.

Název v anglickém jazyce

Yeast and fungal cell-wall polysaccharides can self-assemble in vitro into an ultrastructure resembling in vivo yeast cell walls

Popis výsledku anglicky

Polysaccharides account for more than 90% of the content of fungal cell walls, but the mechanism underlying the formation of the architecture of the cell walls, which consist of microfibrils embedded in an amorphous wall matrix, remains unknown. We usedelectron microscopy to investigate ten different fungal cell-wall polysaccharides to determine whether they could self-assemble into the fibrillar or amorphous component of fungal cell walls in a test tube without enzymes. The ultrastructures formed by precipitating beta-1,3-glucan and beta-1,6-glucan are different depending on the existence of branching in the molecule. Linear beta-1,3- glucan and linear beta-1,6-glucan precipitate into a fibrillar ultrastructure. Branched beta-1,6-glucan, mannan and glycogen precipitates are amorphous. Branched beta-1,3-glucan forms a fibrillar plus amorphous ultrastructure.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microscopy

ISSN

0022-0744

e-ISSN

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Svazek periodika

62

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

327-339

Kód UT WoS článku

000320457100001

EID výsledku v databázi Scopus

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