Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F15%3A00084517" target="_blank" >RIV/00216224:14110/15:00084517 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/15:00063508

Výsledek na webu

<a href="http://dx.doi.org/10.1089/mdr.2014.0210" target="_blank" >http://dx.doi.org/10.1089/mdr.2014.0210</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1089/mdr.2014.0210" target="_blank" >10.1089/mdr.2014.0210</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

Popis výsledku v původním jazyce

The infections caused by extended-spectrum beta-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients? clinical material, was developed. This study focused on blaCTX-M and blaSHV determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M andSHV ESBL enzymes using realtime PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the blaCTX-M. In two samples, the ESBL blaSHV-ESBL gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL

Název v anglickém jazyce

Application of Molecular Diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

Popis výsledku anglicky

The infections caused by extended-spectrum beta-lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients? clinical material, was developed. This study focused on blaCTX-M and blaSHV determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M andSHV ESBL enzymes using realtime PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the blaCTX-M. In two samples, the ESBL blaSHV-ESBL gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables detection of ESBL

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbial Drug Resistance

ISSN

1076-6294

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

352-357

Kód UT WoS článku

000363875600015

EID výsledku v databázi Scopus

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