Application of Molecular diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A73574810" target="_blank" >RIV/61989592:15110/15:73574810 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Application of Molecular diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

Popis výsledku v původním jazyce

The infections caused by extended-spectrum -lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients&apos; clinical material, was developed. This study focused on bla(CTX-M) and bla(SHV) determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using real-time PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the bla(CTX-M). In two samples, the ESBL bla(SHV-ESBL) gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables

Název v anglickém jazyce

Application of Molecular diagnostics in Primary Detection of ESBL Directly from Clinical Specimens

Popis výsledku anglicky

The infections caused by extended-spectrum -lactamase (ESBL)-producing organisms are associated with increased mortality. The real-time polymerase chain reaction (PCR) method, which enables detection of ESBLs directly from patients&apos; clinical material, was developed. This study focused on bla(CTX-M) and bla(SHV) determination in endotracheal aspirates. Each sample was identified with standard microbiological procedures and simultaneously analyzed for the presence of nucleic acids, which encode CTX-M and SHV ESBL enzymes using real-time PCR. A total of 341 samples were investigated. In the set, 27 ESBL-positive samples were identified by phenotypic methods, while 60 positive samples were identified by the PCR method. Of the 60 PCR-positive samples, 58 were positive for the bla(CTX-M). In two samples, the ESBL bla(SHV-ESBL) gene was detected. One phenotypically positive sample was PCR negative. The real-time PCR assay does not require a cultivation step and therefore enables

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

AIMS ENVIRONMENTAL SCIENCE

ISSN

2372-0344

e-ISSN

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Svazek periodika

2015

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

1

Strana od-do

1

Kód UT WoS článku

000363875600015

EID výsledku v databázi Scopus

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