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Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F17%3A00099019" target="_blank" >RIV/00216224:14110/17:00099019 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/62156489:43210/17:43912612

  • Výsledek na webu

  • DOI - Digital Object Identifier

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats

  • Popis výsledku v původním jazyce

    The objective of the present study was to compare gene expression of selected markers depending on the type of diet with additional oil in feeding mixture. The experiment was analysed by the real-time polymerase chain reaction method (RT-PCR). As a housekeeping gene with stabile expression was selected beta-actin gene (Actb) and analysed markers were peroxisome proliferator- activated receptor gamma (PPARgamma), G-protein coupled receptor-sensor (GPR120), adiponectin receptor 1 (Adipor1) and adiponectin receptor 2 (Adipor2). Forty-eight adult male rats (Wistar Albino) were divided into four groups and were fed 10 weeks the diet containing specific oil for each group. Group R was fed by fish oil added to basic feeding mixture, group P was fed by palm oil added to basic mixture, group S was fed by safflower oil added to basic mixture and group D was fed by Schizochytrium microalga extract added to basic mixture. After 10 weeks of feeding, rats were sacrificed by isoflurane overdosing and liver samples were taken and expression of the liver genes coding four selected markers was measured. The main hypothesis of this project was if there were any differences in expression levels among groups depending on the type of diet.

  • Název v anglickém jazyce

    Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats

  • Popis výsledku anglicky

    The objective of the present study was to compare gene expression of selected markers depending on the type of diet with additional oil in feeding mixture. The experiment was analysed by the real-time polymerase chain reaction method (RT-PCR). As a housekeeping gene with stabile expression was selected beta-actin gene (Actb) and analysed markers were peroxisome proliferator- activated receptor gamma (PPARgamma), G-protein coupled receptor-sensor (GPR120), adiponectin receptor 1 (Adipor1) and adiponectin receptor 2 (Adipor2). Forty-eight adult male rats (Wistar Albino) were divided into four groups and were fed 10 weeks the diet containing specific oil for each group. Group R was fed by fish oil added to basic feeding mixture, group P was fed by palm oil added to basic mixture, group S was fed by safflower oil added to basic mixture and group D was fed by Schizochytrium microalga extract added to basic mixture. After 10 weeks of feeding, rats were sacrificed by isoflurane overdosing and liver samples were taken and expression of the liver genes coding four selected markers was measured. The main hypothesis of this project was if there were any differences in expression levels among groups depending on the type of diet.

Klasifikace

  • Druh

    D - Stať ve sborníku

  • CEP obor

  • OECD FORD obor

    30200 - Clinical medicine

Návaznosti výsledku

  • Projekt

  • Návaznosti

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2017

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název statě ve sborníku

    MendelNet 2017 : Proceedings of 24th International PhD Students Conference

  • ISBN

    9788075095299

  • ISSN

  • e-ISSN

  • Počet stran výsledku

    5

  • Strana od-do

    756-760

  • Název nakladatele

    Mendel University in Brno

  • Místo vydání

    Brno

  • Místo konání akce

    Mendel University in Brno, Brno, Czech Republic

  • Datum konání akce

    8. 11. 2017

  • Typ akce podle státní příslušnosti

    WRD - Celosvětová akce

  • Kód UT WoS článku

    000440194500136