Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F17%3A00099019" target="_blank" >RIV/00216224:14110/17:00099019 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43210/17:43912612
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats
Popis výsledku v původním jazyce
The objective of the present study was to compare gene expression of selected markers depending on the type of diet with additional oil in feeding mixture. The experiment was analysed by the real-time polymerase chain reaction method (RT-PCR). As a housekeeping gene with stabile expression was selected beta-actin gene (Actb) and analysed markers were peroxisome proliferator- activated receptor gamma (PPARgamma), G-protein coupled receptor-sensor (GPR120), adiponectin receptor 1 (Adipor1) and adiponectin receptor 2 (Adipor2). Forty-eight adult male rats (Wistar Albino) were divided into four groups and were fed 10 weeks the diet containing specific oil for each group. Group R was fed by fish oil added to basic feeding mixture, group P was fed by palm oil added to basic mixture, group S was fed by safflower oil added to basic mixture and group D was fed by Schizochytrium microalga extract added to basic mixture. After 10 weeks of feeding, rats were sacrificed by isoflurane overdosing and liver samples were taken and expression of the liver genes coding four selected markers was measured. The main hypothesis of this project was if there were any differences in expression levels among groups depending on the type of diet.
Název v anglickém jazyce
Effect of docosahexaenoic (DHA) and eicosapentaenoic acid (EPA) feeding on selected markers expression in rats
Popis výsledku anglicky
The objective of the present study was to compare gene expression of selected markers depending on the type of diet with additional oil in feeding mixture. The experiment was analysed by the real-time polymerase chain reaction method (RT-PCR). As a housekeeping gene with stabile expression was selected beta-actin gene (Actb) and analysed markers were peroxisome proliferator- activated receptor gamma (PPARgamma), G-protein coupled receptor-sensor (GPR120), adiponectin receptor 1 (Adipor1) and adiponectin receptor 2 (Adipor2). Forty-eight adult male rats (Wistar Albino) were divided into four groups and were fed 10 weeks the diet containing specific oil for each group. Group R was fed by fish oil added to basic feeding mixture, group P was fed by palm oil added to basic mixture, group S was fed by safflower oil added to basic mixture and group D was fed by Schizochytrium microalga extract added to basic mixture. After 10 weeks of feeding, rats were sacrificed by isoflurane overdosing and liver samples were taken and expression of the liver genes coding four selected markers was measured. The main hypothesis of this project was if there were any differences in expression levels among groups depending on the type of diet.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
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OECD FORD obor
30200 - Clinical medicine
Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2017
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
MendelNet 2017 : Proceedings of 24th International PhD Students Conference
ISBN
9788075095299
ISSN
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e-ISSN
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Počet stran výsledku
5
Strana od-do
756-760
Název nakladatele
Mendel University in Brno
Místo vydání
Brno
Místo konání akce
Mendel University in Brno, Brno, Czech Republic
Datum konání akce
8. 11. 2017
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000440194500136