Polymorphisms in genes coding purinoreceptor and osteoprotegerin in relation to external apical root resorption in patients after orthodontic treatment

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00100870" target="_blank" >RIV/00216224:14110/18:00100870 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Polymorphisms in genes coding purinoreceptor and osteoprotegerin in relation to external apical root resorption in patients after orthodontic treatment

Popis výsledku v původním jazyce

Background: A bidirectional relationship between chronic periodontitis (CP) and diabetes mellitus type 2 (T2DM), characterized by low-grade inflammation, was previously described. Purinergic signaling plays a role in the activation of multiprotein intracellular complexes called inflammasomes, which control release of potent proinflammatory cytokines. The aim of the present study was to analyze two purinergic receptor (P2RX7) gene variants with gain-of-function effect in patients T2DM and/or CP in Czech population. Subjects and methods: Totally, 473 unrelated subjects were included in this case-control study. Genomic DNA of 208 patients with CP, 83 patients with T2DM+CP and 182 systemically healthy non-periodontitis controls were genotyped using the qPCR TaqMan method for His155Tyr (rs208294, C/T) and Ala348Thr (rs1718119, A/G) polymorphisms in the P2RX7 gene. Results: No significant differences in allele and/or genotype frequencies of P2RX7 His155Tyr between cases and controls were found. However, the G allele and GG genotype of P2RX7 Ala348Thr variant were marginally associated with CP (P=0.065 and P=0.079, respectively). In addition, the GG genotype, encoding Ala/Ala in amino acid sequence, was negatively correlated with levels of glycated hemoglobin (HbA1c) in T2DM patients (P&lt;0.01); patients with Thr/Thr genotype that is associated with gain-of-function had the highest levels of HbA1c. Conclusions: Although only marginal association of polymorphism P2RX7 Ala348Thr with susceptibility to CP in the Czech population was found, purinergic signaling via P2RX7 gene variability might influence glucose regulation.

Název v anglickém jazyce

Polymorphisms in genes coding purinoreceptor and osteoprotegerin in relation to external apical root resorption in patients after orthodontic treatment

Popis výsledku anglicky

Background: A bidirectional relationship between chronic periodontitis (CP) and diabetes mellitus type 2 (T2DM), characterized by low-grade inflammation, was previously described. Purinergic signaling plays a role in the activation of multiprotein intracellular complexes called inflammasomes, which control release of potent proinflammatory cytokines. The aim of the present study was to analyze two purinergic receptor (P2RX7) gene variants with gain-of-function effect in patients T2DM and/or CP in Czech population. Subjects and methods: Totally, 473 unrelated subjects were included in this case-control study. Genomic DNA of 208 patients with CP, 83 patients with T2DM+CP and 182 systemically healthy non-periodontitis controls were genotyped using the qPCR TaqMan method for His155Tyr (rs208294, C/T) and Ala348Thr (rs1718119, A/G) polymorphisms in the P2RX7 gene. Results: No significant differences in allele and/or genotype frequencies of P2RX7 His155Tyr between cases and controls were found. However, the G allele and GG genotype of P2RX7 Ala348Thr variant were marginally associated with CP (P=0.065 and P=0.079, respectively). In addition, the GG genotype, encoding Ala/Ala in amino acid sequence, was negatively correlated with levels of glycated hemoglobin (HbA1c) in T2DM patients (P&lt;0.01); patients with Thr/Thr genotype that is associated with gain-of-function had the highest levels of HbA1c. Conclusions: Although only marginal association of polymorphism P2RX7 Ala348Thr with susceptibility to CP in the Czech population was found, purinergic signaling via P2RX7 gene variability might influence glucose regulation.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30101 - Human genetics

Projekt

<a href="/cs/project/GB14-37368G" target="_blank" >GB14-37368G: Centrum orofaciálního vývoje a regenerace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů