Determination of pyrroloquinoline quinone by capillary zone electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00003262" target="_blank" >RIV/00216224:14310/00:00003262 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of pyrroloquinoline quinone by capillary zone electrophoresis

Popis výsledku v původním jazyce

A new method for the determination of pyrroloquinoline quinone by capillary zone electrophoresis has been developed. Separation conditions have been optimised with the respect to different parameters including pH and ionic strength of the background electrolyte, separation voltage and temperature of the capillary. A buffer consisting of 50 mM b-alanine - HCl pH 3.0 was found to be the most suitable electrolyte for this separation. An applied voltage of 25 kV (negative polarity) and a temperature of 25 oC gave the best analysis of pyrroloquinoline quinone. The linear detection range for concentration versus peak area for the assay is from 5 to 500 mM (correlation coefficient 0.9998) with a detection limit of 0.1- 0.2 mM. The inter-day reproducibility ofthe peak area was 2.5 % and the inter-day reproducibility of the migration time was below 0.18 %.

Název v anglickém jazyce

Determination of pyrroloquinoline quinone by capillary zone electrophoresis

Popis výsledku anglicky

A new method for the determination of pyrroloquinoline quinone by capillary zone electrophoresis has been developed. Separation conditions have been optimised with the respect to different parameters including pH and ionic strength of the background electrolyte, separation voltage and temperature of the capillary. A buffer consisting of 50 mM b-alanine - HCl pH 3.0 was found to be the most suitable electrolyte for this separation. An applied voltage of 25 kV (negative polarity) and a temperature of 25 oC gave the best analysis of pyrroloquinoline quinone. The linear detection range for concentration versus peak area for the assay is from 5 to 500 mM (correlation coefficient 0.9998) with a detection limit of 0.1- 0.2 mM. The inter-day reproducibility ofthe peak area was 2.5 % and the inter-day reproducibility of the migration time was below 0.18 %.

Druh

B - Odborná kniha

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA525%2F97%2F0340" target="_blank" >GA525/97/0340: Aktivita bakterie Thiobacilus ferrooxidans ve vztahu k ekotoxikologii sulfidických odpadů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

ISBN

ISSN 0378-4347

Počet stran knihy

7

Název nakladatele

Elsevier

Místo vydání

Amsterdam

Kód UT WoS knihy

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