Determination of Gentiopicroside in Extracts of Centaurium erythreae and Gentiana lutea by Micellar Electrokinetic Capillary Chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00003302" target="_blank" >RIV/00216224:14310/00:00003302 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of Gentiopicroside in Extracts of Centaurium erythreae and Gentiana lutea by Micellar Electrokinetic Capillary Chromatography

Popis výsledku v původním jazyce

Micellar electrokinetic capillary chromatography has been developed as a promising method for the determination of gentiopicroside. The separation conditions have been optimised with respect to the different parameters including SDS concentration, pH andion strength of the background electrolyte, and temperature of capillary. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.6 was found to be the most suitable electrolyte for this separation. The applied voltage of 28 kV (positive polarity) and the temperature of capillary 20 oC gave the best separation of secoiridiods. The linear detection range for concentration versus peak area for the assay is from 5 to 500 mg . ml-1 (correlation coefficient 0.9998) with a detection limit of 0.3 mg . ml-1. The inter-day reproducibility of the peak area was 2.5 % and the inter-day reproducibility of the migration time was below 0.16 %. The developed method was successfully used to quantify genti

Název v anglickém jazyce

Determination of Gentiopicroside in Extracts of Centaurium erythreae and Gentiana lutea by Micellar Electrokinetic Capillary Chromatography

Popis výsledku anglicky

Micellar electrokinetic capillary chromatography has been developed as a promising method for the determination of gentiopicroside. The separation conditions have been optimised with respect to the different parameters including SDS concentration, pH andion strength of the background electrolyte, and temperature of capillary. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.6 was found to be the most suitable electrolyte for this separation. The applied voltage of 28 kV (positive polarity) and the temperature of capillary 20 oC gave the best separation of secoiridiods. The linear detection range for concentration versus peak area for the assay is from 5 to 500 mg . ml-1 (correlation coefficient 0.9998) with a detection limit of 0.3 mg . ml-1. The inter-day reproducibility of the peak area was 2.5 % and the inter-day reproducibility of the migration time was below 0.16 %. The developed method was successfully used to quantify genti

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA303%2F00%2FD062" target="_blank" >GA303/00/D062: Biologická dostupnost a mechanismus antioxidačního účinku fenolických kyselin a lignanů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

J.Liquid Chromatogr. Rel. Technol.

ISSN

1082-6076

e-ISSN

—

Svazek periodika

23

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—