Dynamika flexibilnych RNA pantov dolezitych pre funkciu ribozomu: ne-Watson-Crick-ovske pary bazi, modularna vystavba a specificka hydratacia RNA Kink-turn motivov

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00009958" target="_blank" >RIV/00216224:14310/04:00009958 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

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Název v původním jazyce

Dynamics of Flexible RNA Hinges Relevant to Ribosome Function: non-Watson-Crick basepairs, modular building and specific hydration of RNA Kink-turns

Popis výsledku v původním jazyce

Hinge-like RNA motifs occur at conserved positions in the 16S and 23S ribosomal RNAs, as revealed by x-ray crystallography of the 50S subunits of H. marismortui and D. radiodurans and the 30S subunit of T. thermophilus. These motifs, asymmetric internalloops, are called Kink-turns (K-turns), and are characterized by a sharp, ca. 120 deg. bend in both phosphodiester backbones producing a V-shaped structure with an acute angle of ca. 60 deg. between the flanking RNA helices. The bend is stabilized by non-Watson-Crick basepairs involving the minor (shallow) grooves of the helices. We have carried out a set of explicit-solvent Molecular Dynamics (MD) simulations for selected K-turn motifs, including K-turn 38 (Kt-38) occurred in the A-site finger, Kt-42 occurred in the Factor-binding domain and Kt-58 occurred in the Domain III from the 23S ribosomal RNA of H. marismortui. The presence of K-turns at these key functional sites of the 50S subunit of the ribosome suggests that they confer fle

Název v anglickém jazyce

Dynamics of Flexible RNA Hinges Relevant to Ribosome Function: non-Watson-Crick basepairs, modular building and specific hydration of RNA Kink-turns

Popis výsledku anglicky

Hinge-like RNA motifs occur at conserved positions in the 16S and 23S ribosomal RNAs, as revealed by x-ray crystallography of the 50S subunits of H. marismortui and D. radiodurans and the 30S subunit of T. thermophilus. These motifs, asymmetric internalloops, are called Kink-turns (K-turns), and are characterized by a sharp, ca. 120 deg. bend in both phosphodiester backbones producing a V-shaped structure with an acute angle of ca. 60 deg. between the flanking RNA helices. The bend is stabilized by non-Watson-Crick basepairs involving the minor (shallow) grooves of the helices. We have carried out a set of explicit-solvent Molecular Dynamics (MD) simulations for selected K-turn motifs, including K-turn 38 (Kt-38) occurred in the A-site finger, Kt-42 occurred in the Factor-binding domain and Kt-58 occurred in the Domain III from the 23S ribosomal RNA of H. marismortui. The presence of K-turns at these key functional sites of the 50S subunit of the ribosome suggests that they confer fle

Druh

D - Stať ve sborníku

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/LN00A016" target="_blank" >LN00A016: BIOMOLEKULÁRNÍ CENTRUM</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Materials Structure in Chemistry, Biology, Physics and Technology

ISBN

ISSN 1211-5894

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

28

Název nakladatele

Krystalografická společnost

Místo vydání

Praha

Místo konání akce

Academic and University Center, Nové Hrady

Datum konání akce

11. 3. 2004

Typ akce podle státní příslušnosti

CST - Celostátní akce

Kód UT WoS článku

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