Indirect quantification of protein modification degree

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00014091" target="_blank" >RIV/00216224:14310/05:00014091 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Indirect quantification of protein modification degree

Popis výsledku v původním jazyce

In our work, we present a general, indirect method, using which it is possible to quantify the percentage of the given amino acid modified in total in a protein sequence (protein modification degree, PMD). From the knowledge of the identity of amino acidresidues modifiable during post-translation process, PMD and its changes during time of particular amino acid residue can be determined. Such a quantification can be achieved by means of easy stable isotope modification (via 18O-labelling by trypsination) and consequential MALDI TOF MS analysis.

Název v anglickém jazyce

Indirect quantification of protein modification degree

Popis výsledku anglicky

In our work, we present a general, indirect method, using which it is possible to quantify the percentage of the given amino acid modified in total in a protein sequence (protein modification degree, PMD). From the knowledge of the identity of amino acidresidues modifiable during post-translation process, PMD and its changes during time of particular amino acid residue can be determined. Such a quantification can be achieved by means of easy stable isotope modification (via 18O-labelling by trypsination) and consequential MALDI TOF MS analysis.

Druh

O - Ostatní výsledky

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů