Elektrochemický imunosensor pro detekci Francisella tularensis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F05%3A00015144" target="_blank" >RIV/00216224:14310/05:00015144 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00000493:G41__/05:#0000032

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical immunosensor for detection of Francisella tularensis

Popis výsledku v původním jazyce

The immunosensor for rapid and sensitive detection of Francisella tularensis (Ft) was developed using screen-printed sensors with multiple (4) Au working electrodes. The sensing zones were modified with a biolayer of anti-Ft polyclonal antibody attachedthrough the covalently immobilized Protein A. The sandwich assay format was employed with anti-Ft IgG-peroxidase conjugate as tracer. For electrochemical measurements, an automated portable detector was developed; it consisted of a digitally controlled potentiostat and four embedded miniperistaltic pumps. The flow-through measurement involved sequential incubations with sample (10 min), tracer (10 min) and substrate mixture (iodide and hydrogen peroxide). The enzymatically-produced iodine was detected electrochemically at - 50 mV. The limit of detection was 100 Ft cells/ml; the response was significantly higher (3x) when using the living cells compared to the heat inactivated ones.

Název v anglickém jazyce

Electrochemical immunosensor for detection of Francisella tularensis

Popis výsledku anglicky

The immunosensor for rapid and sensitive detection of Francisella tularensis (Ft) was developed using screen-printed sensors with multiple (4) Au working electrodes. The sensing zones were modified with a biolayer of anti-Ft polyclonal antibody attachedthrough the covalently immobilized Protein A. The sandwich assay format was employed with anti-Ft IgG-peroxidase conjugate as tracer. For electrochemical measurements, an automated portable detector was developed; it consisted of a digitally controlled potentiostat and four embedded miniperistaltic pumps. The flow-through measurement involved sequential incubations with sample (10 min), tracer (10 min) and substrate mixture (iodide and hydrogen peroxide). The enzymatically-produced iodine was detected electrochemically at - 50 mV. The limit of detection was 100 Ft cells/ml; the response was significantly higher (3x) when using the living cells compared to the heat inactivated ones.

Druh

C - Kapitola v odborné knize

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/OBVTUO2003001" target="_blank" >OBVTUO2003001: PROTILÁTKY 2 - Imunosenzory bojových chemických látek a bojových biologických prostředků</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Defense against bioterror

ISBN

1-4020-3385-0

Počet stran výsledku

12

Strana od-do

221-232

Počet stran knihy

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Název nakladatele

Springer

Místo vydání

Dordrecht, The Netherlands

Kód UT WoS kapitoly

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