Chemically functionalized AFM tips: easy way to resolution enhancement in single biomolecule visualization.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F08%3A00052276" target="_blank" >RIV/00216224:14310/08:00052276 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Chemically functionalized AFM tips: easy way to resolution enhancement in single biomolecule visualization.

Popis výsledku v původním jazyce

Atomic Force Microscopy (AFM) technique allows visualization of structures in a sub-nanometer scale, i.e. imaging of individual biomolecules. In principle AFM scanning probe, presented by ultra-sharp tip, is in close contact with surface and relief is visualized as 3D map (after conversion of a measured quantity, e.g. tip bending). Resolution of an AFM microscope is essentially affected by the tip curvature (sharpness), however other experimental conditions would affect this parameter, too. Only weak and medium forces are usually employed in the interaction between tip and surface in the relief visualization. Surfaces visualized in a non-controlled atmosphere are naturally coated with a very thin (1-5 nm) layer of adsorbed water. In some cases capillary forces originating from this layer can strongly affect visualization process, as those forces are much stronger comparing to other intermolecular forces (van derWaals, hydrogen, ionic forces, etc.).

Název v anglickém jazyce

Chemically functionalized AFM tips: easy way to resolution enhancement in single biomolecule visualization.

Popis výsledku anglicky

Atomic Force Microscopy (AFM) technique allows visualization of structures in a sub-nanometer scale, i.e. imaging of individual biomolecules. In principle AFM scanning probe, presented by ultra-sharp tip, is in close contact with surface and relief is visualized as 3D map (after conversion of a measured quantity, e.g. tip bending). Resolution of an AFM microscope is essentially affected by the tip curvature (sharpness), however other experimental conditions would affect this parameter, too. Only weak and medium forces are usually employed in the interaction between tip and surface in the relief visualization. Surfaces visualized in a non-controlled atmosphere are naturally coated with a very thin (1-5 nm) layer of adsorbed water. In some cases capillary forces originating from this layer can strongly affect visualization process, as those forces are much stronger comparing to other intermolecular forces (van derWaals, hydrogen, ionic forces, etc.).

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/2B06056" target="_blank" >2B06056: Diagnostika poškození DNA polyaromatickými sloučeninami použitím nanotechnologických a bioanalytických metod pro včasnou detekci karcinomu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

5th international interdisciplinary meeting on bioanalysis CECE 2008

ISBN

978-80-254-3194-8

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

65-66

Název nakladatele

Institute of Analytical Chemisty AS CR

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

24. 11. 2008

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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