c-Myb promotes motility and invasion of breast cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00040519" target="_blank" >RIV/00216224:14310/10:00040519 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/10:00040584

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

c-Myb promotes motility and invasion of breast cancer cells

Popis výsledku v původním jazyce

The aim of this study was to assess the role of the c-Myb protein in control of invasion of breast cancer cells. MDA-MB-231 breast cancer cells were transfected with the c-Myb coding cDNA to prepare MDA-MB-231MYBup derivatives. The effects of c-Myb overexpression on migration and invasion capacity of these cells were assessed. MDA-MB-231MYBup cells were significantly more active in both motility and invasion than controls. In order to reveal dynamics of these processes, we performed real-time analysis of cell migration and invasion using the xCELLigence RT-CA system. This analysis clearly confirmed increased invasive capacity of MDA-MB-231MYBup cells compared to controls. To address the mechanism of c-Myb-enhanced breast cancer cell invasion, we examined the role of c-Myb in control of expression and activity of some of the proteases involved in degradation of extracellular matrix. We found that c-Myb increased production of cathepsin D and matrix metalloproteinases in MDA-MB-231MYBup

Název v anglickém jazyce

c-Myb promotes motility and invasion of breast cancer cells

Popis výsledku anglicky

The aim of this study was to assess the role of the c-Myb protein in control of invasion of breast cancer cells. MDA-MB-231 breast cancer cells were transfected with the c-Myb coding cDNA to prepare MDA-MB-231MYBup derivatives. The effects of c-Myb overexpression on migration and invasion capacity of these cells were assessed. MDA-MB-231MYBup cells were significantly more active in both motility and invasion than controls. In order to reveal dynamics of these processes, we performed real-time analysis of cell migration and invasion using the xCELLigence RT-CA system. This analysis clearly confirmed increased invasive capacity of MDA-MB-231MYBup cells compared to controls. To address the mechanism of c-Myb-enhanced breast cancer cell invasion, we examined the role of c-Myb in control of expression and activity of some of the proteases involved in degradation of extracellular matrix. We found that c-Myb increased production of cathepsin D and matrix metalloproteinases in MDA-MB-231MYBup

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů