The c-Myb protein promotes motility and invasion of breast cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00040584" target="_blank" >RIV/00216224:14310/10:00040584 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The c-Myb protein promotes motility and invasion of breast cancer cells

Popis výsledku v původním jazyce

c-Myb is a key regulator of progenitor cells in bone marrow, colonic crypts and adult brain. Activation of c-myb is associated with leukemias and solid tumors. The aim of this study was to assess the role of the c-Myb protein in control of invasion of breast cancer cells. c-Myb overexpression enhanced migration and invasiveness of MDA-MB-231 cells as determined by transwell cell invasion assay and real-time analysis of cell migration and invasion using the xCELLigence RTCA system. qRT-PCR analysis revealed c-Myb-dependent upregulation of the genes coding for proteases that are involved in invasive behavior of MDA-MB-231 cells: increased production of cathepsin D and matrix metalloproteinase 9 (MMP9) in MDA-MB-231MYBup cells were further confirmed by western blotting. Transactivation assay revealed that c-Myb can activate transcription from the cathepsin D promoter. This denotes cathepsin D as a novel Myb-target gene. c-Myb promotes motility and invasivity of breast cancer MDA-MB-231 ce

Název v anglickém jazyce

The c-Myb protein promotes motility and invasion of breast cancer cells

Popis výsledku anglicky

c-Myb is a key regulator of progenitor cells in bone marrow, colonic crypts and adult brain. Activation of c-myb is associated with leukemias and solid tumors. The aim of this study was to assess the role of the c-Myb protein in control of invasion of breast cancer cells. c-Myb overexpression enhanced migration and invasiveness of MDA-MB-231 cells as determined by transwell cell invasion assay and real-time analysis of cell migration and invasion using the xCELLigence RTCA system. qRT-PCR analysis revealed c-Myb-dependent upregulation of the genes coding for proteases that are involved in invasive behavior of MDA-MB-231 cells: increased production of cathepsin D and matrix metalloproteinase 9 (MMP9) in MDA-MB-231MYBup cells were further confirmed by western blotting. Transactivation assay revealed that c-Myb can activate transcription from the cathepsin D promoter. This denotes cathepsin D as a novel Myb-target gene. c-Myb promotes motility and invasivity of breast cancer MDA-MB-231 ce

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů