c-Myb-induced migration and invasiveness of breast and colon cancer cells are associated with up-regulation of cathepsin D

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F10%3A00040620" target="_blank" >RIV/00216224:14310/10:00040620 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

c-Myb-induced migration and invasiveness of breast and colon cancer cells are associated with up-regulation of cathepsin D

Popis výsledku v původním jazyce

c-Myb is essential regulator of stem-progenitor cells in bone marrow, colon epithelia and neurogenic niches in brain. Malfunction of c-Myb results in malignancies of several types including breast and colon cancer. Its role in control of invasion and metastasis of tumor cells remains unclear. This study shows that overexpession of c-Myb enhances ability of human breast cancer MDA-MB-231 cells and mouse colon cancer CT26 cells to migrate and invade Matrigel in vitro. Real-time analysis of cell migrationand invasion using the xCELLigence RTCA system revealed dynamics of c-Myb-induced effects with rapid onset of migration and invasion during first 6 and 12 hours, respectively. Real-time PCR screening revealed up-regulation of cathepsin D and matrix metalloproteinase 9 (MMP9) mRNAs coding for proteases that promote invasive behavior in c-Myb-overexpressing cells. Further experiments confirmed that increased production of cathepsin D and MMP 9 correlate with c-Myb-induced invasivity of MDA

Název v anglickém jazyce

c-Myb-induced migration and invasiveness of breast and colon cancer cells are associated with up-regulation of cathepsin D

Popis výsledku anglicky

c-Myb is essential regulator of stem-progenitor cells in bone marrow, colon epithelia and neurogenic niches in brain. Malfunction of c-Myb results in malignancies of several types including breast and colon cancer. Its role in control of invasion and metastasis of tumor cells remains unclear. This study shows that overexpession of c-Myb enhances ability of human breast cancer MDA-MB-231 cells and mouse colon cancer CT26 cells to migrate and invade Matrigel in vitro. Real-time analysis of cell migrationand invasion using the xCELLigence RTCA system revealed dynamics of c-Myb-induced effects with rapid onset of migration and invasion during first 6 and 12 hours, respectively. Real-time PCR screening revealed up-regulation of cathepsin D and matrix metalloproteinase 9 (MMP9) mRNAs coding for proteases that promote invasive behavior in c-Myb-overexpressing cells. Further experiments confirmed that increased production of cathepsin D and MMP 9 correlate with c-Myb-induced invasivity of MDA

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů