Measurements of DNA base composition with flow cytometry and DNA temperature melting

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00050575" target="_blank" >RIV/00216224:14310/11:00050575 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Measurements of DNA base composition with flow cytometry and DNA temperature melting

Popis výsledku v původním jazyce

Base composition (expressed most frequently as GC content) is an important classification criterion in microbial research. This might be also the case of plants, however, the data on GC contents in plants are still very poor and some skepticism still exist on the reliability of older estimates as well as on the accuracy of recently cumulating data from flow cytometry (FCM) experiments. FCM enables to measure GC content by comparing results with base specific and base unspecific dyes, most frequently unspecific PI and AT-specific DAPI (Barow et Meister, 2002; Bureš et al., 2004; Šmarda et al., 2008). This measurements are critically dependent on the knowledge of binding length of the used base specific dye (i.e., the number of consecutive pairs of the same type enabling binding of a dye molecule), which is, however, mostly uncertain for many dyes and may vary with specific base patterning in a natural DNA.

Název v anglickém jazyce

Measurements of DNA base composition with flow cytometry and DNA temperature melting

Popis výsledku anglicky

Base composition (expressed most frequently as GC content) is an important classification criterion in microbial research. This might be also the case of plants, however, the data on GC contents in plants are still very poor and some skepticism still exist on the reliability of older estimates as well as on the accuracy of recently cumulating data from flow cytometry (FCM) experiments. FCM enables to measure GC content by comparing results with base specific and base unspecific dyes, most frequently unspecific PI and AT-specific DAPI (Barow et Meister, 2002; Bureš et al., 2004; Šmarda et al., 2008). This measurements are critically dependent on the knowledge of binding length of the used base specific dye (i.e., the number of consecutive pairs of the same type enabling binding of a dye molecule), which is, however, mostly uncertain for many dyes and may vary with specific base patterning in a natural DNA.

Druh

O - Ostatní výsledky

CEP obor

EF - Botanika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů