Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00053555" target="_blank" >RIV/00216224:14310/11:00053555 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62156489:43210/11:00173505 RIV/00216305:26220/11:PU96271
Výsledek na webu
<a href="http://ac.els-cdn.com/S0956566310006494/1-s2.0-S0956566310006494-main.pdf?_tid=45582c491f72810960ab2a3968016b45&acdnat=1332250805_449357aac5e95feac50ebc201b6b9175" target="_blank" >http://ac.els-cdn.com/S0956566310006494/1-s2.0-S0956566310006494-main.pdf?_tid=45582c491f72810960ab2a3968016b45&acdnat=1332250805_449357aac5e95feac50ebc201b6b9175</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bios.2010.09.035" target="_blank" >10.1016/j.bios.2010.09.035</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver.
Popis výsledku v původním jazyce
In this paper, heavy metal biosensor based on immobilization of metallothionein (MT) to the surface of carbon paste electrode (CPE) via anti-MT-antibodies is reported. First, the evaluation of MT electroactivity was done. The attention was focused on thecapturing of MT to the CPE surface. Antibodies incorporated and mixed into carbon paste were stable; even after two weeks the observed changes in signal height were lower than 5%. Further, the interaction of MT with polyclonal chicken antibodies incorporated in carbon paste electrode was determined by square-wave voltammetry. In the voltammogram, two signals - labelled as cys(MT) and W(a) - were observed. The cyst,AT corresponded to -SH moieties of MT and W(a) corresponded to tryptophan residues of chicken antibodies. Time of interaction (300 s) and MT concentration (125 mu g/ml) were optimized to suggest a silver(I) ions biosensor.
Název v anglickém jazyce
Immobilization of metallothionein to carbon paste electrode surface via anti-MT antibodies and its use for biosensing of silver.
Popis výsledku anglicky
In this paper, heavy metal biosensor based on immobilization of metallothionein (MT) to the surface of carbon paste electrode (CPE) via anti-MT-antibodies is reported. First, the evaluation of MT electroactivity was done. The attention was focused on thecapturing of MT to the CPE surface. Antibodies incorporated and mixed into carbon paste were stable; even after two weeks the observed changes in signal height were lower than 5%. Further, the interaction of MT with polyclonal chicken antibodies incorporated in carbon paste electrode was determined by square-wave voltammetry. In the voltammogram, two signals - labelled as cys(MT) and W(a) - were observed. The cyst,AT corresponded to -SH moieties of MT and W(a) corresponded to tryptophan residues of chicken antibodies. Time of interaction (300 s) and MT concentration (125 mu g/ml) were optimized to suggest a silver(I) ions biosensor.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CF - Fyzikální chemie a teoretická chemie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
BIOSENSORS & BIOELECTRONICS
ISSN
0956-5663
e-ISSN
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Svazek periodika
26
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
7
Strana od-do
2201-2207
Kód UT WoS článku
000286904400066
EID výsledku v databázi Scopus
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