The variability and expression of MHC genes and their rolein host-parasite interactions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F12%3A00058592" target="_blank" >RIV/00216224:14310/12:00058592 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The variability and expression of MHC genes and their rolein host-parasite interactions

Popis výsledku v původním jazyce

The contribution of immunoecological studies has gained a place of central importance in recent evolutionary biology and ecology. The main aim of the work is to analyze the associations between multiple parasites and host immunocompetence and to investigate the structure and evolutionary mechanism of selection in MHC genes of cyprinid fish species. The extremely high level of allelic diversity in the genes of major histocompatibility complex results of many mechanisms and is determined with the intent to obtain genetic data for evolutionary and population studies. Nowadays, for MHC genotyping we use two basic approaches: 454 clonal pyrosequencing of amplicons and CE-SSCP of amplicon clones combined with automatic sequencing based on Sanger technology.In this contribution, we present primary results of a new approach for MHC genes variability tests ? high resolution melting analysis (HRM) and expression studies (using qPCR), that enable us to extend the studies on MHC genes up to funct

Název v anglickém jazyce

The variability and expression of MHC genes and their rolein host-parasite interactions

Popis výsledku anglicky

The contribution of immunoecological studies has gained a place of central importance in recent evolutionary biology and ecology. The main aim of the work is to analyze the associations between multiple parasites and host immunocompetence and to investigate the structure and evolutionary mechanism of selection in MHC genes of cyprinid fish species. The extremely high level of allelic diversity in the genes of major histocompatibility complex results of many mechanisms and is determined with the intent to obtain genetic data for evolutionary and population studies. Nowadays, for MHC genotyping we use two basic approaches: 454 clonal pyrosequencing of amplicons and CE-SSCP of amplicon clones combined with automatic sequencing based on Sanger technology.In this contribution, we present primary results of a new approach for MHC genes variability tests ? high resolution melting analysis (HRM) and expression studies (using qPCR), that enable us to extend the studies on MHC genes up to funct

Druh

O - Ostatní výsledky

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GBP505%2F12%2FG112" target="_blank" >GBP505/12/G112: ECIP - Evropské centrum ichtyoparazitologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů