HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F13%3A00070150" target="_blank" >RIV/00216224:14310/13:00070150 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts

Popis výsledku v původním jazyce

Predominant medical effect of many plants and herbs is related to the presence of polyphenolic secondary plant metabolites commonly known as flavonoids. Different classes of flavonoids are synthesized as a response to the ambient stimuli (climate, stress, mold attacks etc.) and accumulate in various cells and plant parts. The antioxidant potential and selected flavonoids content in sea buckthorn (Hippophaë Rhamnoides L.) plant part materials (stems, leaves and fruits) has been investigated during one vegetative cycle. New RP-HPLC method using gradient elution for determination of 10 polyphenols, namely catechin, epicatechin, rutin, quercetin, quercitrin, kaempferol, gallic acid, p-coumaric acid, caffeic acid and ferulic acid has been developed. Significant part of method development included optimization of extraction step for sample matrix removal. As the most suitable, liquid-liquid extraction of methanolic plant solutions using diethylether was found.

Název v anglickém jazyce

HPLC profiles of polyphenolic compounds and estimation of antioxidant potential in sea buckthorn plant parts

Popis výsledku anglicky

Predominant medical effect of many plants and herbs is related to the presence of polyphenolic secondary plant metabolites commonly known as flavonoids. Different classes of flavonoids are synthesized as a response to the ambient stimuli (climate, stress, mold attacks etc.) and accumulate in various cells and plant parts. The antioxidant potential and selected flavonoids content in sea buckthorn (Hippophaë Rhamnoides L.) plant part materials (stems, leaves and fruits) has been investigated during one vegetative cycle. New RP-HPLC method using gradient elution for determination of 10 polyphenols, namely catechin, epicatechin, rutin, quercetin, quercitrin, kaempferol, gallic acid, p-coumaric acid, caffeic acid and ferulic acid has been developed. Significant part of method development included optimization of extraction step for sample matrix removal. As the most suitable, liquid-liquid extraction of methanolic plant solutions using diethylether was found.

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů