Comparison of DNA extraction methods in terms of yield, purity, long-term storage and downstream manipulation with brewer's yeast chromosomal DNA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00075066" target="_blank" >RIV/00216224:14310/14:00075066 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/14:00435775 RIV/60193697:_____/14:#0000965

Výsledek na webu

<a href="http://dx.doi.org/10.1094/ASBCJ-2014-0110-01" target="_blank" >http://dx.doi.org/10.1094/ASBCJ-2014-0110-01</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1094/ASBCJ-2014-0110-01" target="_blank" >10.1094/ASBCJ-2014-0110-01</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of DNA extraction methods in terms of yield, purity, long-term storage and downstream manipulation with brewer's yeast chromosomal DNA

Popis výsledku v původním jazyce

Five methods of DNA isolation (a ?classical? phenol/chloroform extraction and four commercially available kits) were compared in terms of the yield, purity, integrity and stability of extracted DNA from both laboratory and industrial yeast strains. DNA isolation with commercial kits employing silica based adsorption collumns mostly gave lower yields of DNA, integrity of DNA soon declines and after six months of storage under the manufacturer's instructions the DNA was almost fully degraded. Isolation ofDNA by using bead breaking of cells resulted in very low yield and low purity of the DNA also. The use of a commercial kit without silica collumns and phenol/chloroform treatment as well as the traditional phenol/chloroform extraction were certainly more time-consuming, but the extracted DNA was of high purity and concentration, with minimal damage, stable over months and usable for subsequent molecular analyses (cloning, PCR, RFLP, DNA sequencing).

Název v anglickém jazyce

Comparison of DNA extraction methods in terms of yield, purity, long-term storage and downstream manipulation with brewer's yeast chromosomal DNA

Popis výsledku anglicky

Five methods of DNA isolation (a ?classical? phenol/chloroform extraction and four commercially available kits) were compared in terms of the yield, purity, integrity and stability of extracted DNA from both laboratory and industrial yeast strains. DNA isolation with commercial kits employing silica based adsorption collumns mostly gave lower yields of DNA, integrity of DNA soon declines and after six months of storage under the manufacturer's instructions the DNA was almost fully degraded. Isolation ofDNA by using bead breaking of cells resulted in very low yield and low purity of the DNA also. The use of a commercial kit without silica collumns and phenol/chloroform treatment as well as the traditional phenol/chloroform extraction were certainly more time-consuming, but the extracted DNA was of high purity and concentration, with minimal damage, stable over months and usable for subsequent molecular analyses (cloning, PCR, RFLP, DNA sequencing).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The Journal of the American Society of Brewing Chemists

ISSN

0361-0470

e-ISSN

—

Svazek periodika

72

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

5

Strana od-do

1-5

Kód UT WoS článku

000332594500001

EID výsledku v databázi Scopus

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