Comparison and optimalization of DNA extraction methods using new magnetic particles for analysis of Haloarcula

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F17%3APU124175" target="_blank" >RIV/00216305:26310/17:PU124175 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison and optimalization of DNA extraction methods using new magnetic particles for analysis of Haloarcula

Popis výsledku v původním jazyce

Haloarcula used in this study was previously isolated from salt evaporation ponds belonging to Salinas del Odiel S.L. Company, located in the natural reserve of Odiel marshlands in the Southwest Spain1. DNA extraction is an essential step in all molecular biologist diagnostic methods. We compare three used methods, and found their DNA was changed in quality and quantity. A fundamental challenge in using these approaches has been to isolate DNA that is representative of the Haloarcula culture. Three different DNA extraction, performed with commercial kit (GeneJET Genomic DNA Purification Kit. Thermo Scientific, Wilmington, USA), a standard phenol-chloroform method and two magnetic micro- and nanoparticles were evaluated, using the addition of Haloarcula cells. DNA extracts were analysed by agarose gel electrophoresis and UV spectroscopy. Two different types of magnetic particles, prepared by Horák in Institute of Macromolecular Chemistry AS CR, were used for solid - phase DNA extraction. First types o

Název v anglickém jazyce

Comparison and optimalization of DNA extraction methods using new magnetic particles for analysis of Haloarcula

Popis výsledku anglicky

Haloarcula used in this study was previously isolated from salt evaporation ponds belonging to Salinas del Odiel S.L. Company, located in the natural reserve of Odiel marshlands in the Southwest Spain1. DNA extraction is an essential step in all molecular biologist diagnostic methods. We compare three used methods, and found their DNA was changed in quality and quantity. A fundamental challenge in using these approaches has been to isolate DNA that is representative of the Haloarcula culture. Three different DNA extraction, performed with commercial kit (GeneJET Genomic DNA Purification Kit. Thermo Scientific, Wilmington, USA), a standard phenol-chloroform method and two magnetic micro- and nanoparticles were evaluated, using the addition of Haloarcula cells. DNA extracts were analysed by agarose gel electrophoresis and UV spectroscopy. Two different types of magnetic particles, prepared by Horák in Institute of Macromolecular Chemistry AS CR, were used for solid - phase DNA extraction. First types o

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů