Cysteine peptidase inhibitors of Trichinella spiralis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00084265" target="_blank" >RIV/00216224:14310/15:00084265 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cysteine peptidase inhibitors of Trichinella spiralis

Popis výsledku v původním jazyce

Trichinella spriralis is well known causative agent of human disease trichinellosis. Individuals of this nematode parasite produce various types of cysteine peptidase inhibitors (cystatins) in order to regulate the function of cysteine peptidases and modulate the immune response of the host organism. Therefore we aim to reveal the differences in production of cystatin by different T. spiralis developmental stages. Three types of cystatins were identified in the genome of T. spiralis cystatin B, onchocystatin and multi cystatin domain protein. ICR and SCID mice were experimentally infected per os with infective muscle stage larvae (ML) of T. spiralis. Preadults (Ad3), adults (Ad5), new born larvae (NBL) and muscle stage (ML) larvae of T. spiralis were isolated from the intestinal and muscle tissue at various time post infection. Excretory/secretory products of all developmental stages were isolated and screened for the presence of cystatins using mass spectrometry.

Název v anglickém jazyce

Cysteine peptidase inhibitors of Trichinella spiralis

Popis výsledku anglicky

Trichinella spriralis is well known causative agent of human disease trichinellosis. Individuals of this nematode parasite produce various types of cysteine peptidase inhibitors (cystatins) in order to regulate the function of cysteine peptidases and modulate the immune response of the host organism. Therefore we aim to reveal the differences in production of cystatin by different T. spiralis developmental stages. Three types of cystatins were identified in the genome of T. spiralis cystatin B, onchocystatin and multi cystatin domain protein. ICR and SCID mice were experimentally infected per os with infective muscle stage larvae (ML) of T. spiralis. Preadults (Ad3), adults (Ad5), new born larvae (NBL) and muscle stage (ML) larvae of T. spiralis were isolated from the intestinal and muscle tissue at various time post infection. Excretory/secretory products of all developmental stages were isolated and screened for the presence of cystatins using mass spectrometry.

Druh

O - Ostatní výsledky

CEP obor

EG - Zoologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů