Multimode fibre: a pathway towards deep tissue fluorescence microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00116094" target="_blank" >RIV/00216224:14310/15:00116094 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1117/12.2202355" target="_blank" >https://doi.org/10.1117/12.2202355</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1117/12.2202355" target="_blank" >10.1117/12.2202355</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Multimode fibre: a pathway towards deep tissue fluorescence microscopy

Popis výsledku v původním jazyce

Fluorescence microscopy has emerged as a pivotal platform for imaging in the life sciences. In recent years, the overwhelming success of its different modalities has been accompanied by various efforts to carry out imaging deeper inside living tissues. A key challenge of these efforts is to overcome scattering and absorption of light in such environments. Multiple strategies (e.g. multi-photon, wavefront correction techniques) extended the penetration depth to the current state-of-the-art of about 1000 mu m at the resolution of approximately 1 mu m. The only viable strategy for imaging deeper than this is by employing a fibre bundle based endoscope. However, such devices lack resolution and have a significant footprint (1mm in diameter), which prohibits their use in studies involving tissues deep in live animals. We have recently demonstrated a radically new approach that delivers the light in/out of place of interest through an extremely thin (tens of microns in diameter) cylindrical glass tube called a multimode optical fibre (MMF). Not only is this type of delivery much less invasive compared to fibre bundle technology, it also enables higher resolution and has the ability to image at any plane behind the fibre without any auxiliary optics. The two most important limitations of this exciting technology are (i) the lack of bending flexibility and (ii) high demands on computational power, making the performance of such systems slow. We will discuss how to overcome these limitations.

Název v anglickém jazyce

Multimode fibre: a pathway towards deep tissue fluorescence microscopy

Popis výsledku anglicky

Fluorescence microscopy has emerged as a pivotal platform for imaging in the life sciences. In recent years, the overwhelming success of its different modalities has been accompanied by various efforts to carry out imaging deeper inside living tissues. A key challenge of these efforts is to overcome scattering and absorption of light in such environments. Multiple strategies (e.g. multi-photon, wavefront correction techniques) extended the penetration depth to the current state-of-the-art of about 1000 mu m at the resolution of approximately 1 mu m. The only viable strategy for imaging deeper than this is by employing a fibre bundle based endoscope. However, such devices lack resolution and have a significant footprint (1mm in diameter), which prohibits their use in studies involving tissues deep in live animals. We have recently demonstrated a radically new approach that delivers the light in/out of place of interest through an extremely thin (tens of microns in diameter) cylindrical glass tube called a multimode optical fibre (MMF). Not only is this type of delivery much less invasive compared to fibre bundle technology, it also enables higher resolution and has the ability to image at any plane behind the fibre without any auxiliary optics. The two most important limitations of this exciting technology are (i) the lack of bending flexibility and (ii) high demands on computational power, making the performance of such systems slow. We will discuss how to overcome these limitations.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MICRO+NANO MATERIALS, DEVICES, AND SYSTEMS

ISBN

9781628418903

ISSN

0277-786X

e-ISSN

1996-756X

Počet stran výsledku

6

Strana od-do

1-6

Název nakladatele

SPIE-INT SOC OPTICAL ENGINEERING

Místo vydání

BELLINGHAM

Místo konání akce

Sydney, AUSTRALIA

Datum konání akce

6. 12. 2015

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000370723500052