Multimode fibre: a pathway towards deep tissue fluorescence microscopy
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F15%3A00116094" target="_blank" >RIV/00216224:14310/15:00116094 - isvavai.cz</a>
Výsledek na webu
<a href="https://doi.org/10.1117/12.2202355" target="_blank" >https://doi.org/10.1117/12.2202355</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1117/12.2202355" target="_blank" >10.1117/12.2202355</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Multimode fibre: a pathway towards deep tissue fluorescence microscopy
Popis výsledku v původním jazyce
Fluorescence microscopy has emerged as a pivotal platform for imaging in the life sciences. In recent years, the overwhelming success of its different modalities has been accompanied by various efforts to carry out imaging deeper inside living tissues. A key challenge of these efforts is to overcome scattering and absorption of light in such environments. Multiple strategies (e.g. multi-photon, wavefront correction techniques) extended the penetration depth to the current state-of-the-art of about 1000 mu m at the resolution of approximately 1 mu m. The only viable strategy for imaging deeper than this is by employing a fibre bundle based endoscope. However, such devices lack resolution and have a significant footprint (1mm in diameter), which prohibits their use in studies involving tissues deep in live animals. We have recently demonstrated a radically new approach that delivers the light in/out of place of interest through an extremely thin (tens of microns in diameter) cylindrical glass tube called a multimode optical fibre (MMF). Not only is this type of delivery much less invasive compared to fibre bundle technology, it also enables higher resolution and has the ability to image at any plane behind the fibre without any auxiliary optics. The two most important limitations of this exciting technology are (i) the lack of bending flexibility and (ii) high demands on computational power, making the performance of such systems slow. We will discuss how to overcome these limitations.
Název v anglickém jazyce
Multimode fibre: a pathway towards deep tissue fluorescence microscopy
Popis výsledku anglicky
Fluorescence microscopy has emerged as a pivotal platform for imaging in the life sciences. In recent years, the overwhelming success of its different modalities has been accompanied by various efforts to carry out imaging deeper inside living tissues. A key challenge of these efforts is to overcome scattering and absorption of light in such environments. Multiple strategies (e.g. multi-photon, wavefront correction techniques) extended the penetration depth to the current state-of-the-art of about 1000 mu m at the resolution of approximately 1 mu m. The only viable strategy for imaging deeper than this is by employing a fibre bundle based endoscope. However, such devices lack resolution and have a significant footprint (1mm in diameter), which prohibits their use in studies involving tissues deep in live animals. We have recently demonstrated a radically new approach that delivers the light in/out of place of interest through an extremely thin (tens of microns in diameter) cylindrical glass tube called a multimode optical fibre (MMF). Not only is this type of delivery much less invasive compared to fibre bundle technology, it also enables higher resolution and has the ability to image at any plane behind the fibre without any auxiliary optics. The two most important limitations of this exciting technology are (i) the lack of bending flexibility and (ii) high demands on computational power, making the performance of such systems slow. We will discuss how to overcome these limitations.
Klasifikace
Druh
D - Stať ve sborníku
CEP obor
—
OECD FORD obor
10306 - Optics (including laser optics and quantum optics)
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název statě ve sborníku
MICRO+NANO MATERIALS, DEVICES, AND SYSTEMS
ISBN
9781628418903
ISSN
0277-786X
e-ISSN
1996-756X
Počet stran výsledku
6
Strana od-do
1-6
Název nakladatele
SPIE-INT SOC OPTICAL ENGINEERING
Místo vydání
BELLINGHAM
Místo konání akce
Sydney, AUSTRALIA
Datum konání akce
6. 12. 2015
Typ akce podle státní příslušnosti
WRD - Celosvětová akce
Kód UT WoS článku
000370723500052