New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F17%3A00098646" target="_blank" >RIV/00216224:14310/17:00098646 - isvavai.cz</a>

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria

Popis výsledku v původním jazyce

Different genera of sulfate-reducing bacteria (SRB) are always detected in the large intestine of humans and animals with diseases like an ulcerative colitis and Crohn's disease or even cancer. The final metabolism product of these anaerobic microorganisms is hydrogen sulfide which is known as a toxic substance and can lead to damage of epithelial cells of the bowel in high concentration. Some genera of the intestinal SRB included to the Desulfovibrionaceae family are hard to cultivate or even uncultivable. Isolation of these genera is also complicated because there are others satellite microorganisms. Up to now, Postgate’s medium and other media do still not solve the cultivation problem and are created generally for Desulfovibrio species from nature environment but not for SRB species from the intestine. The object of our research was to modify the principle of isolation of intestinal SRB and cultivation medium based on their physiological and biochemical properties. Thus, there is no selective medium for intestine SRB which would improve cultivation and isolation of these important microorganisms. New created medium can be useful for more opportunities of intestinal SRB cultivation and understanding their involvement in inflammatory bowel diseases.

Název v anglickém jazyce

New modification of cultivation medium for isolation and growth of intestinal sulfate-reducing bacteria

Popis výsledku anglicky

Different genera of sulfate-reducing bacteria (SRB) are always detected in the large intestine of humans and animals with diseases like an ulcerative colitis and Crohn's disease or even cancer. The final metabolism product of these anaerobic microorganisms is hydrogen sulfide which is known as a toxic substance and can lead to damage of epithelial cells of the bowel in high concentration. Some genera of the intestinal SRB included to the Desulfovibrionaceae family are hard to cultivate or even uncultivable. Isolation of these genera is also complicated because there are others satellite microorganisms. Up to now, Postgate’s medium and other media do still not solve the cultivation problem and are created generally for Desulfovibrio species from nature environment but not for SRB species from the intestine. The object of our research was to modify the principle of isolation of intestinal SRB and cultivation medium based on their physiological and biochemical properties. Thus, there is no selective medium for intestine SRB which would improve cultivation and isolation of these important microorganisms. New created medium can be useful for more opportunities of intestinal SRB cultivation and understanding their involvement in inflammatory bowel diseases.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10600 - Biological sciences

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2017 Proceedings of 24th International PhD Students Conference

ISBN

9788075095299

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

702-707

Název nakladatele

Faculty of AgriSciences, Mendel University in Brno

Místo vydání

Brno, Czech Republic

Místo konání akce

Brno

Datum konání akce

8. 11. 2017

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000440194500125