Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00107222" target="_blank" >RIV/00216224:14310/19:00107222 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201800367" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201800367</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201800367" target="_blank" >10.1002/elps.201800367</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques

Popis výsledku v původním jazyce

Nearly all processes in living organisms are controlled and regulated by the synergy of many biomolecule interactions involving proteins, peptides, nucleic acids, nucleotides, saccharides and small molecular weight ligands. There is growing interest in understanding them, not only for the purposes of interactomics as an essential part of system biology, but also in their further elucidation in disease pathology, diagnostics and treatment. The necessity of detailed investigation of these interactions leads to the requirement of laboratory methods characterised by high efficiency and sensitivity. As a result, many instrumental approaches differing in their fundamental principles have been developed, including those based on capillary electrophoresis. Although capillary electrophoresis offers numerous advantages for such studies, it still has one serious limitation, its poor concentration sensitivity with the most commonly used detection method – ultraviolet-visible spectrometry. However, coupling capillary electrophoresis with a more sensitive detector fulfils the above-mentioned requirement. In this review, capillary electrophoresis combined with fluorescence, mass spectrometry and several non-traditional detection techniques in affinity interaction studies are summarised and discussed, together with the possibility of conducting these measurements in microchip format.

Název v anglickém jazyce

Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques

Popis výsledku anglicky

Nearly all processes in living organisms are controlled and regulated by the synergy of many biomolecule interactions involving proteins, peptides, nucleic acids, nucleotides, saccharides and small molecular weight ligands. There is growing interest in understanding them, not only for the purposes of interactomics as an essential part of system biology, but also in their further elucidation in disease pathology, diagnostics and treatment. The necessity of detailed investigation of these interactions leads to the requirement of laboratory methods characterised by high efficiency and sensitivity. As a result, many instrumental approaches differing in their fundamental principles have been developed, including those based on capillary electrophoresis. Although capillary electrophoresis offers numerous advantages for such studies, it still has one serious limitation, its poor concentration sensitivity with the most commonly used detection method – ultraviolet-visible spectrometry. However, coupling capillary electrophoresis with a more sensitive detector fulfils the above-mentioned requirement. In this review, capillary electrophoresis combined with fluorescence, mass spectrometry and several non-traditional detection techniques in affinity interaction studies are summarised and discussed, together with the possibility of conducting these measurements in microchip format.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10600 - Biological sciences

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

1522-2683

Svazek periodika

40

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

18

Strana od-do

625-642

Kód UT WoS článku

000460305100004

EID výsledku v databázi Scopus

2-s2.0-85059838110