Selected viral, protozoan and bacterial agents on minimally processed vegetables and sprouts at point of sale

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F21%3A00122001" target="_blank" >RIV/00216224:14310/21:00122001 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/21:N0000096

Výsledek na webu

<a href="https://www.vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=60&article=2226" target="_blank" >https://www.vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=60&article=2226</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Selected viral, protozoan and bacterial agents on minimally processed vegetables and sprouts at point of sale

Popis výsledku v původním jazyce

Epidemiological data linked to cases of food-borne infection from fresh produce are scarce and usually underestimated. The present study reports the occurrence of food-borne pathogens such as viruses (norovirus, hepatitis A virus ) and protozoa (Giardia intestinalis, Ctyptosporidiunt parmmlhominis) in 175 samples of fresh-cut vegetables, frozen vegetables and sprouts that originated in the Czech Republic and in other European countries, as detected using realtime PCR and reverse transcription real-time PCR. In addition, samples were analysed for Escherichia coli as an indicator of fecal contamination and for Staphylococcus aureus as an indicator of possible contamination by food handlers, using conventional culture methods. Norovirus was detected in two samples (1.1 %) of rocket and frozen mixed vegetables. G. intestinalis was detected in two samples (1.1 %) of mixed fresh vegetable salads and mixed frozen vegetables. The occurrence of norovirus did not correlate with E. coil presence. Staph. aureus was detected in 3 samples (1.7 %) but without genes for classical enterotoxins production. Our results highlight the need for microbiological analysis of food of non-animal origin for specific protozoan and viral agents, which are being less frequently monitored in these food category types, but their presence may pose risk for consumers.

Název v anglickém jazyce

Selected viral, protozoan and bacterial agents on minimally processed vegetables and sprouts at point of sale

Popis výsledku anglicky

Epidemiological data linked to cases of food-borne infection from fresh produce are scarce and usually underestimated. The present study reports the occurrence of food-borne pathogens such as viruses (norovirus, hepatitis A virus ) and protozoa (Giardia intestinalis, Ctyptosporidiunt parmmlhominis) in 175 samples of fresh-cut vegetables, frozen vegetables and sprouts that originated in the Czech Republic and in other European countries, as detected using realtime PCR and reverse transcription real-time PCR. In addition, samples were analysed for Escherichia coli as an indicator of fecal contamination and for Staphylococcus aureus as an indicator of possible contamination by food handlers, using conventional culture methods. Norovirus was detected in two samples (1.1 %) of rocket and frozen mixed vegetables. G. intestinalis was detected in two samples (1.1 %) of mixed fresh vegetable salads and mixed frozen vegetables. The occurrence of norovirus did not correlate with E. coil presence. Staph. aureus was detected in 3 samples (1.7 %) but without genes for classical enterotoxins production. Our results highlight the need for microbiological analysis of food of non-animal origin for specific protozoan and viral agents, which are being less frequently monitored in these food category types, but their presence may pose risk for consumers.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food and Nutrition Research

ISSN

1336-8672

e-ISSN

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Svazek periodika

60

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

8

Strana od-do

138-145

Kód UT WoS článku

000667177300005

EID výsledku v databázi Scopus

2-s2.0-85110243457