The microbiological quality of bacteriophage product and its influence on antibacterial phage effect

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F23%3A00132456" target="_blank" >RIV/00216224:14310/23:00132456 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The microbiological quality of bacteriophage product and its influence on antibacterial phage effect

Popis výsledku v původním jazyce

Developing a drug form containing bacteriophages is an essential step of phage therapy implementation in practice. A crucial criterion of the final phage product is phage stability in variant conditions, in which applied phages could be exposed during manipulation with the product (i.e., contaminations by microorganisms and drug additives). This work concerns phage titer stability during exposition to preservatives, yeast, and mold. We assessed the stability of a myovirus against Pseudomonas aeruginosa and a myovirus against Staphylococcus aureus. These purified phages were inoculated by Candida albicans or Aspergillus niger and stored at room temperature in six variant preservatives, including organic acids salts and alcohols. After 24 hours, the titer of phages was determined by a standard drop test.

Název v anglickém jazyce

The microbiological quality of bacteriophage product and its influence on antibacterial phage effect

Popis výsledku anglicky

Developing a drug form containing bacteriophages is an essential step of phage therapy implementation in practice. A crucial criterion of the final phage product is phage stability in variant conditions, in which applied phages could be exposed during manipulation with the product (i.e., contaminations by microorganisms and drug additives). This work concerns phage titer stability during exposition to preservatives, yeast, and mold. We assessed the stability of a myovirus against Pseudomonas aeruginosa and a myovirus against Staphylococcus aureus. These purified phages were inoculated by Candida albicans or Aspergillus niger and stored at room temperature in six variant preservatives, including organic acids salts and alcohols. After 24 hours, the titer of phages was determined by a standard drop test.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30403 - Technologies involving identifying the functioning of DNA, proteins and enzymes and how they influence the onset of disease and maintenance of well-being (gene-based diagnostics and therapeutic interventions [pharmacogenomics, gene-based therapeutics])

Projekt

<a href="/cs/project/LX22NPO5103" target="_blank" >LX22NPO5103: Národní institut virologie a bakteriologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů