Induction of somatic embryogenesis and ectopic proliferation in Tecoma stans (L.) Juss. ex Kunth cell suspension culture

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F24%3A00135836" target="_blank" >RIV/00216224:14310/24:00135836 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11627-024-10421-4" target="_blank" >https://link.springer.com/article/10.1007/s11627-024-10421-4</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11627-024-10421-4" target="_blank" >10.1007/s11627-024-10421-4</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Induction of somatic embryogenesis and ectopic proliferation in Tecoma stans (L.) Juss. ex Kunth cell suspension culture

Popis výsledku v původním jazyce

Somatic embryogenesis is a developmental pathway where somatic cells of plants generate embryogenic cells that subsequently mature into somatic embryos under favorable conditions. This process is one of the most important in vitro techniques for plant propagation, with diverse practical implications. In this study, ectopic proliferation and somatic embryos from Tecoma stans (L.) Juss. ex Kunth cell cultures were induced by employing primary conditioning Murashige and Skoog medium supplemented with 1.0 mg L−1 2,4-dichlorophenoxyacetic acid. Subsequently, a secondary induction medium supplemented with a combination of 1.0 mg L−1 2,4-dichlorophenoxyacetic acid with various concentrations of 6-benzyladenine cytokinin (1 to 5 mg L−1) was used to promote embryogenesis. The results revealed the successful formation of pre-embryonic and embryonic stages, including globular, heart, torpedo, and cotyledon stages within a 2-wk incubation period under the specified hormonal conditions, leading to subsequent development into the mature vegetative phase after an additional 4 wk. Significant embryo production (16 ± 2.0 torpedo stage embryos per 50 mL culture media) was observed in Murashige and Skoog medium enriched with 1.0 mg L−1 2,4-dichlorophenoxyacetic acid and 2.0 mg L−1 6-benzyladenine, surpassing the results observed with other concentrations (p-value &lt; 0.0001). The generated somatic embryos can serve as a potential in vitro tool for the propagation, generation, and organogenesis of T. stans, contributing to its role as both an ornamental and medicinal plant. Moreover, the induction of somatic embryogenesis opens avenues for the potential production of T. stans bioactive secondary metabolites and diverse applications in biotechnology, biotransformation, and biocatalysis, particularly in the conversion of both exogenous and endogenous substrates, such as tecomine—the principal antidiabetic alkaloid in the leaf extract.

Název v anglickém jazyce

Induction of somatic embryogenesis and ectopic proliferation in Tecoma stans (L.) Juss. ex Kunth cell suspension culture

Popis výsledku anglicky

Somatic embryogenesis is a developmental pathway where somatic cells of plants generate embryogenic cells that subsequently mature into somatic embryos under favorable conditions. This process is one of the most important in vitro techniques for plant propagation, with diverse practical implications. In this study, ectopic proliferation and somatic embryos from Tecoma stans (L.) Juss. ex Kunth cell cultures were induced by employing primary conditioning Murashige and Skoog medium supplemented with 1.0 mg L−1 2,4-dichlorophenoxyacetic acid. Subsequently, a secondary induction medium supplemented with a combination of 1.0 mg L−1 2,4-dichlorophenoxyacetic acid with various concentrations of 6-benzyladenine cytokinin (1 to 5 mg L−1) was used to promote embryogenesis. The results revealed the successful formation of pre-embryonic and embryonic stages, including globular, heart, torpedo, and cotyledon stages within a 2-wk incubation period under the specified hormonal conditions, leading to subsequent development into the mature vegetative phase after an additional 4 wk. Significant embryo production (16 ± 2.0 torpedo stage embryos per 50 mL culture media) was observed in Murashige and Skoog medium enriched with 1.0 mg L−1 2,4-dichlorophenoxyacetic acid and 2.0 mg L−1 6-benzyladenine, surpassing the results observed with other concentrations (p-value &lt; 0.0001). The generated somatic embryos can serve as a potential in vitro tool for the propagation, generation, and organogenesis of T. stans, contributing to its role as both an ornamental and medicinal plant. Moreover, the induction of somatic embryogenesis opens avenues for the potential production of T. stans bioactive secondary metabolites and diverse applications in biotechnology, biotransformation, and biocatalysis, particularly in the conversion of both exogenous and endogenous substrates, such as tecomine—the principal antidiabetic alkaloid in the leaf extract.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

In Vitro Cellular & Developmental Biology - Plant

ISSN

1054-5476

e-ISSN

1475-2689

Svazek periodika

60

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

396-402

Kód UT WoS článku

001204131000001

EID výsledku v databázi Scopus

2-s2.0-85190765405