Determination of lamotrigine unbound fraction using ultrafiltration and validated LC-MS method
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F24%3A00139973" target="_blank" >RIV/00216224:14310/24:00139973 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Determination of lamotrigine unbound fraction using ultrafiltration and validated LC-MS method
Popis výsledku v původním jazyce
Total plasma concentrations are commonly used for therapeutic drug monitoring of antiepileptic drugs (AED). On the other hand, drug activity and at least some of adverse effects depend on the unbound fraction of a drug in the bloodstream. The determination of free (unbound) concentrations by ultrafiltration is expected to reflect the effective drug concentration. Further, saliva is considered as a plasma ultrafiltrate, thus referring to free drug concentration. Therefore, the aim was to develop a method for the measurement of total and unbound AED concentrations in plasma and correlate them to saliva AED concentration. As a model drug, lamotrigine (LTG) was chosen since it is one of the most widely used AED and is moderately bound to albumin (~55%) [1]. Pilot data showed that the concentrations measured in plasma ultrafiltrate and saliva were in close proximity, even though total plasma concentrations are much higher and wildly differ between individual samples. More data must be available to determine the correlation between saliva and plasma concentrations and saliva and ultrafiltrate concentrations.
Název v anglickém jazyce
Determination of lamotrigine unbound fraction using ultrafiltration and validated LC-MS method
Popis výsledku anglicky
Total plasma concentrations are commonly used for therapeutic drug monitoring of antiepileptic drugs (AED). On the other hand, drug activity and at least some of adverse effects depend on the unbound fraction of a drug in the bloodstream. The determination of free (unbound) concentrations by ultrafiltration is expected to reflect the effective drug concentration. Further, saliva is considered as a plasma ultrafiltrate, thus referring to free drug concentration. Therefore, the aim was to develop a method for the measurement of total and unbound AED concentrations in plasma and correlate them to saliva AED concentration. As a model drug, lamotrigine (LTG) was chosen since it is one of the most widely used AED and is moderately bound to albumin (~55%) [1]. Pilot data showed that the concentrations measured in plasma ultrafiltrate and saliva were in close proximity, even though total plasma concentrations are much higher and wildly differ between individual samples. More data must be available to determine the correlation between saliva and plasma concentrations and saliva and ultrafiltrate concentrations.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])
Návaznosti výsledku
Projekt
<a href="/cs/project/NU23-08-00229" target="_blank" >NU23-08-00229: Minimálně invazivní metody pro personalizovanou farmakoterapii epilepsie</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2024
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů