Novel multipurpose matrix for a high resolution MALDI MS and multimodal imaging

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F25%3A00144507" target="_blank" >RIV/00216224:14310/25:00144507 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Novel multipurpose matrix for a high resolution MALDI MS and multimodal imaging

Popis výsledku v původním jazyce

The staining with basic fuchsin streamlines sample preparation, eliminating the need for matrix spraying or sublimation, and enables true multimodal analysis on a single tissue section thanks to its compatibility with all employed imaging modalities. Innate fluorescence of basic fuchsin ensuresthat no additional sample treatment or addition of other fluorophores is needed for recording fluorescence images. The technique is demonstrated on mapping of mouse brain: MALDI MSI modality visualizes spatial distribution of lipids and fluorescence combined with histology clearly defines the different areas and tissue types in the brain section. Furthermore, MALDI MS with basic fuchsin is usable with laser postionization, i.e. MALDI-2, to increase sensitivity and spatial resolution to 5 micrometers. The high-quality of recorded images is comparable to the MALDI/MALDI-2 MSI with classical matrices. The developed multimodal workflow facilitates high-resolution molecular imaging and offers tools for holistic characterization of biological tissues.

Název v anglickém jazyce

Novel multipurpose matrix for a high resolution MALDI MS and multimodal imaging

Popis výsledku anglicky

The staining with basic fuchsin streamlines sample preparation, eliminating the need for matrix spraying or sublimation, and enables true multimodal analysis on a single tissue section thanks to its compatibility with all employed imaging modalities. Innate fluorescence of basic fuchsin ensuresthat no additional sample treatment or addition of other fluorophores is needed for recording fluorescence images. The technique is demonstrated on mapping of mouse brain: MALDI MSI modality visualizes spatial distribution of lipids and fluorescence combined with histology clearly defines the different areas and tissue types in the brain section. Furthermore, MALDI MS with basic fuchsin is usable with laser postionization, i.e. MALDI-2, to increase sensitivity and spatial resolution to 5 micrometers. The high-quality of recorded images is comparable to the MALDI/MALDI-2 MSI with classical matrices. The developed multimodal workflow facilitates high-resolution molecular imaging and offers tools for holistic characterization of biological tissues.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA24-10924S" target="_blank" >GA24-10924S: Digitální multiplexní zobrazovací hmotnostní spektrometrie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů