Effect of preoperative apheresis on the amount of apoptotic cells in peripheral blood of pigs during cardiopulmonary bypass

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F99%3A00003588" target="_blank" >RIV/00216224:14310/99:00003588 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of preoperative apheresis on the amount of apoptotic cells in peripheral blood of pigs during cardiopulmonary bypass

Popis výsledku v původním jazyce

Cardiopulmonary bypass (CPB) causes the postperfusion syndrom.The aim of our work was to show if the preoperative apheresis can lead to the decrease of apoptotic cells in blood. The presence of apoptotic cells was followed according to DNA isolated fromgranulocytes, lymfocytes and cells captured on apheretic filter. The experiment showed that apoptotic DNA was present in leucocytes of peripheral blood. However, the estimation of DNA fragmentation was negative in blood cells captured on the filter. It follows that preoperative apheresis can not markedly influence the amount of apioptotic cells in peripheral blood of pigs with CPB. Apoptosis was detected at the beginning of operation in cells of peripheral blood. The apoptotic response can be assumed inconsequence of the stress situation.

Název v anglickém jazyce

Effect of preoperative apheresis on the amount of apoptotic cells in peripheral blood of pigs during cardiopulmonary bypass

Popis výsledku anglicky

Cardiopulmonary bypass (CPB) causes the postperfusion syndrom.The aim of our work was to show if the preoperative apheresis can lead to the decrease of apoptotic cells in blood. The presence of apoptotic cells was followed according to DNA isolated fromgranulocytes, lymfocytes and cells captured on apheretic filter. The experiment showed that apoptotic DNA was present in leucocytes of peripheral blood. However, the estimation of DNA fragmentation was negative in blood cells captured on the filter. It follows that preoperative apheresis can not markedly influence the amount of apioptotic cells in peripheral blood of pigs with CPB. Apoptosis was detected at the beginning of operation in cells of peripheral blood. The apoptotic response can be assumed inconsequence of the stress situation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

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Svazek periodika

48

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

1

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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