Apoptosis in pig leucocytes during cardiopulmonary bypass

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F99%3A00004085" target="_blank" >RIV/00216224:14310/99:00004085 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Apoptosis in pig leucocytes during cardiopulmonary bypass

Popis výsledku v původním jazyce

The aim of this study was to observe programmed cell death (PCD) activation in pig leucocytes durind cardiopulmonary bypass (CPB). Blood samples were taken prior to, during, and after CPB. Apoptosis was detected using TUNEL assay and by typical apoptoticmorphology changes (Giemsa staining). Apoptotic DNA fragmentation was proved in high percentage of cells in the operating beginning. A decrease in the number of apoptotic cells was detected in the surviving pigs after the aorta clip release. On the other hand, the number of apoptotic leucocytes did not significantly change in pigs died owing to postperfusion syndrom. The same alterations in percentage of apoptotic cells were proved in bone marrow after chest opening, after surgery and in dead animals.These results suggested that PCD can play a role in the postperfusion syndrome after CPB.

Název v anglickém jazyce

Apoptosis in pig leucocytes during cardiopulmonary bypass

Popis výsledku anglicky

The aim of this study was to observe programmed cell death (PCD) activation in pig leucocytes durind cardiopulmonary bypass (CPB). Blood samples were taken prior to, during, and after CPB. Apoptosis was detected using TUNEL assay and by typical apoptoticmorphology changes (Giemsa staining). Apoptotic DNA fragmentation was proved in high percentage of cells in the operating beginning. A decrease in the number of apoptotic cells was detected in the surviving pigs after the aorta clip release. On the other hand, the number of apoptotic leucocytes did not significantly change in pigs died owing to postperfusion syndrom. The same alterations in percentage of apoptotic cells were proved in bone marrow after chest opening, after surgery and in dead animals.These results suggested that PCD can play a role in the postperfusion syndrome after CPB.

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/IZ1924" target="_blank" >IZ1924: Postperfuzní syndrom II.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Inflammatory Paradigms and the Vasculature

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

34

Název nakladatele

Keystone Symposia

Místo vydání

New Mexico, USA

Místo konání akce

New Mexico, USA

Datum konání akce

1. 1. 1999

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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