Development of software for automated micro-axialtomography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F02%3A00006218" target="_blank" >RIV/00216224:14330/02:00006218 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of software for automated micro-axialtomography

Popis výsledku v původním jazyce

Micro-axialtomography allows the observation of cells, sub-cellular organelles and their compounds (such as genetic loci or proteins) from an arbitrary angle of view. This is accomplished by means of fixation of the objects to a thin glass fibre and rotation of the fibre around its axis perpendicularly to the optical axis. The cells and their compounds are typically stained by immuno-fluorescence techniques as for instance FISH to label chromatin targets in intact cell nuclei. The rotated objects can beobserved in a fluorescence microscope. In order to facilitate the acquisition and analysis of a large number of objects, the development of an appropriate software module has begun that is incorporated into the image acquisition and analysis package FISH 2.0. Currently the module contains trial versions of the algorithms for image acquisition and analysis and the whole system is being tested. Examples of images obtained using micro-axialtomography are presented as well as the descriptio

Název v anglickém jazyce

Development of software for automated micro-axialtomography

Popis výsledku anglicky

Micro-axialtomography allows the observation of cells, sub-cellular organelles and their compounds (such as genetic loci or proteins) from an arbitrary angle of view. This is accomplished by means of fixation of the objects to a thin glass fibre and rotation of the fibre around its axis perpendicularly to the optical axis. The cells and their compounds are typically stained by immuno-fluorescence techniques as for instance FISH to label chromatin targets in intact cell nuclei. The rotated objects can beobserved in a fluorescence microscope. In order to facilitate the acquisition and analysis of a large number of objects, the development of an appropriate software module has begun that is incorporated into the image acquisition and analysis package FISH 2.0. Currently the module contains trial versions of the algorithms for image acquisition and analysis and the whole system is being tested. Examples of images obtained using micro-axialtomography are presented as well as the descriptio

Druh

D - Stať ve sborníku

CEP obor

JD - Využití počítačů, robotika a její aplikace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Biophysics of the genome and its interactions

ISBN

80-210-2853-X

ISSN

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e-ISSN

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Počet stran výsledku

9

Strana od-do

45

Název nakladatele

Masaryk University

Místo vydání

Brno

Místo konání akce

15-17.10.2001, Hlohovec

Datum konání akce

1. 1. 2001

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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