Autofocusing software issues in automated fluorescence microscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F09%3A00042694" target="_blank" >RIV/00216224:14330/09:00042694 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Autofocusing software issues in automated fluorescence microscopy

Popis výsledku v původním jazyce

This work aims at presenting the basic issues with autofocusing software for automated fluorescence microscopy, together with possible solutions. Namely, three key issues and two techniques are discussed, and the difference between confocal and wide-field microscopy is covered in each case. There are two key factors in image autofocusing: the focus function and the autofocusing algorithm. The algorithm steps along the z-axis and evaluates focus values of each acquired image according to the focus function, in order to find its maximum. In reality, the objects often do not lie in one plane, which results in multimodality of the focus function. Thus all the significant local maxima of this function should be found. Moreover, the peaks can sometimes be coalesced together, especially if they are close to each other, or if one of them is too dominant. This contribution suggests solutions to the following: 1.

Název v anglickém jazyce

Autofocusing software issues in automated fluorescence microscopy

Popis výsledku anglicky

This work aims at presenting the basic issues with autofocusing software for automated fluorescence microscopy, together with possible solutions. Namely, three key issues and two techniques are discussed, and the difference between confocal and wide-field microscopy is covered in each case. There are two key factors in image autofocusing: the focus function and the autofocusing algorithm. The algorithm steps along the z-axis and evaluates focus values of each acquired image according to the focus function, in order to find its maximum. In reality, the objects often do not lie in one plane, which results in multimodality of the focus function. Thus all the significant local maxima of this function should be found. Moreover, the peaks can sometimes be coalesced together, especially if they are close to each other, or if one of them is too dominant. This contribution suggests solutions to the following: 1.

Druh

O - Ostatní výsledky

CEP obor

JD - Využití počítačů, robotika a její aplikace

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů