Genes of the microRNA biogenesis pathway are deregulated in colorectal cancer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00065633" target="_blank" >RIV/00216224:14740/13:00065633 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genes of the microRNA biogenesis pathway are deregulated in colorectal cancer

Popis výsledku v původním jazyce

MicroRNAs (miRNAs) are small non-coding RNAs 18-25 nucleotides long that regulate gene expression on post-transcriptional level. Therefore, their production and maturation have to be strictly regulated. Their biogenesis starts with the transcription, which is followed by several steps that lead to processing of primary transcripts to mature miRNAs. Each step of this pathway is sophistically regulated and any disruption of control mechanisms may leads to the cancer occurrence. The aim of this study was to analyse the expression of the crucial genes involved in the biogenesis of miRNAs in colorectal cancer (CRC). Expression of 19 selected genes (EIF2C1-4, GEMIN4, DDX20, TARBP2, DICER1, XPO5, DROSHA, DGCR8, POLR2A, DDX5, DDX17, ADAR, ADARB1, TNRC6A, LIN28A/B) has been analysed in tumour tissues of 120 clinically characterized patients with CRC and in 120 parallel healthy tissues by qRT-PCR. Using Wilcoxon test, genes with different expression between tumour tissue and healthy tissue have

Název v anglickém jazyce

Genes of the microRNA biogenesis pathway are deregulated in colorectal cancer

Popis výsledku anglicky

MicroRNAs (miRNAs) are small non-coding RNAs 18-25 nucleotides long that regulate gene expression on post-transcriptional level. Therefore, their production and maturation have to be strictly regulated. Their biogenesis starts with the transcription, which is followed by several steps that lead to processing of primary transcripts to mature miRNAs. Each step of this pathway is sophistically regulated and any disruption of control mechanisms may leads to the cancer occurrence. The aim of this study was to analyse the expression of the crucial genes involved in the biogenesis of miRNAs in colorectal cancer (CRC). Expression of 19 selected genes (EIF2C1-4, GEMIN4, DDX20, TARBP2, DICER1, XPO5, DROSHA, DGCR8, POLR2A, DDX5, DDX17, ADAR, ADARB1, TNRC6A, LIN28A/B) has been analysed in tumour tissues of 120 clinically characterized patients with CRC and in 120 parallel healthy tissues by qRT-PCR. Using Wilcoxon test, genes with different expression between tumour tissue and healthy tissue have

Druh

O - Ostatní výsledky

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/NT13549" target="_blank" >NT13549: Vytvoření diagnostické sady cirkulujících mikroRNA pro neinvazivní časnou diagnostiku a sledování pacientů s kolorektálním karcinomem</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů