Fast sampling mode for MALDI TOF mass spectrometry imaging using scanning desorption laser beam

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F13%3A00066034" target="_blank" >RIV/00216224:14740/13:00066034 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fast sampling mode for MALDI TOF mass spectrometry imaging using scanning desorption laser beam

Popis výsledku v původním jazyce

Successful application of matrix-assisted laser desorption/ionization time-of-flight (MALDI TOF) mass spectrometry imaging (MSI) at biomarker discovery, drug and metabolites distribution analysis and neural network studies provided this technique with aninfluential position among imaging techniques. Using label-free approach, MSI is able to detect and localize a wide range of ionizable substances in biological tissues. A disadvantage of this technique is speed of the analysis. Since the very beginning,a single acquisition of a high-resolution MS image could take even days. Recent commercial mass spectrometers employing high-repetition rate solid-state desorption lasers significantly improved MALDI MSI throughput, but the analysis time is still in theorder of hours. Here we introduce a novel sampling approach taking advantage of incorporation of a high-speed precision scanning mirror redirecting the desorption laser beam across the imaged sample area.

Název v anglickém jazyce

Fast sampling mode for MALDI TOF mass spectrometry imaging using scanning desorption laser beam

Popis výsledku anglicky

Successful application of matrix-assisted laser desorption/ionization time-of-flight (MALDI TOF) mass spectrometry imaging (MSI) at biomarker discovery, drug and metabolites distribution analysis and neural network studies provided this technique with aninfluential position among imaging techniques. Using label-free approach, MSI is able to detect and localize a wide range of ionizable substances in biological tissues. A disadvantage of this technique is speed of the analysis. Since the very beginning,a single acquisition of a high-resolution MS image could take even days. Recent commercial mass spectrometers employing high-repetition rate solid-state desorption lasers significantly improved MALDI MSI throughput, but the analysis time is still in theorder of hours. Here we introduce a novel sampling approach taking advantage of incorporation of a high-speed precision scanning mirror redirecting the desorption laser beam across the imaged sample area.

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů