Molecular variability in adult and childhood epileptogenesis: differences in miRNA profile of adult and infantile rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F17%3A00094992" target="_blank" >RIV/00216224:14740/17:00094992 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular variability in adult and childhood epileptogenesis: differences in miRNA profile of adult and infantile rats

Popis výsledku v původním jazyce

Purpose: The onset of epilepsy frequently occurs in early childhood when brain is more susceptible to seizures. The early onset epilepsies are different from those acquired in adulthood. The primary aim of this study was to identify the alterations in microRNA (miRNA/miR) expression in hippocampal tissue of rats undergoing epileptogenesis triggered with LiCl/pilocarpine-induced status epilepticus (SE) in adulthood (P60) or infancy (P12). Method: Rats were exposed to LiCl/pilocarpine induced SE at P60 (n=11) or P12 (P=13). Age matched animals were used as controls (n=11 per each age group). Hippocampal tissue was collected 24h after SE and quickly frozen. Total RNA was isolated from frozen specimens. To address the clinically relevant miRNAs we performed the expression analysis using miQPCR on 30 miRNAs identified with altered expression in mTLE+HS patients. Results: The majority of tested miRNAs had similar expression in both adult and P12 hippocampi. miR-144-5p was downregulated in rats with SE induced in P12 compared with controls. On the other hand, expression levels of let-7i-5p and miR-4443 were decreased only in adult rat brains after SE. Conclusion: Epileptogenesis in adult and developing brain has common features however there are differences on both clinical and molecular level. miR-144-5p, -4443 and let-7i-5p are the possible sources of this variability.

Název v anglickém jazyce

Molecular variability in adult and childhood epileptogenesis: differences in miRNA profile of adult and infantile rats

Popis výsledku anglicky

Purpose: The onset of epilepsy frequently occurs in early childhood when brain is more susceptible to seizures. The early onset epilepsies are different from those acquired in adulthood. The primary aim of this study was to identify the alterations in microRNA (miRNA/miR) expression in hippocampal tissue of rats undergoing epileptogenesis triggered with LiCl/pilocarpine-induced status epilepticus (SE) in adulthood (P60) or infancy (P12). Method: Rats were exposed to LiCl/pilocarpine induced SE at P60 (n=11) or P12 (P=13). Age matched animals were used as controls (n=11 per each age group). Hippocampal tissue was collected 24h after SE and quickly frozen. Total RNA was isolated from frozen specimens. To address the clinically relevant miRNAs we performed the expression analysis using miQPCR on 30 miRNAs identified with altered expression in mTLE+HS patients. Results: The majority of tested miRNAs had similar expression in both adult and P12 hippocampi. miR-144-5p was downregulated in rats with SE induced in P12 compared with controls. On the other hand, expression levels of let-7i-5p and miR-4443 were decreased only in adult rat brains after SE. Conclusion: Epileptogenesis in adult and developing brain has common features however there are differences on both clinical and molecular level. miR-144-5p, -4443 and let-7i-5p are the possible sources of this variability.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30100 - Basic medicine

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů