miRNA sequencing shows different gene expression in adult and infantile epilepsy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F17%3A00094993" target="_blank" >RIV/00216224:14740/17:00094993 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

miRNA sequencing shows different gene expression in adult and infantile epilepsy

Popis výsledku v původním jazyce

Epilepsy is a severe neurological disorder characterized by recurrent seizures. The onset of seizure activity frequently occurs in infancy and early childhood. During the early life period, the neuronal networks are not fully functional, and novel neuronal connections are rapidly formed. Hence, the seizures might have different impact on the adult and developing brain. It was previously shown that the rats with epilepsy onset in the infancy suffer from less severe brain damage later in life than the rats with epilepsy onset in the adulthood. Altered microRNA (short noncoding RNA; miRNA) profile has been associated with epilepsy in numerous studies dealing with both epileptic patients and animal models. To address the differences in gene expression regulation in epilepsy with onset in infancy and adulthood, we performed the analysis of miRNA profiles in infant (12 days old) and adult rats. miRNA profiles were analyzed in the hippocampus (epilepsy onset zone) of male Wistar rats with chemically induced status epilepticus (SE). Tissue samples were collected at two timepoints: 24 hours (acute phase) and 3 months after SE (chronic phase with spontaneous seizures). Sequencing analysis showed that the dysregulation of multiple miRNAs was concordant in both infantile and adult rats in acute phase. Interestingly, numerous miRNAs which were dysregulated in acute phase showed the similar trend in chronic phase in rats with SE in adulthood but not in rats with SE during infancy. These results show that the epilepsy onset in infancy and adulthood alters the regulation of gene expression which might indicate different pathological processes and necessary adjustment of treatment.

Název v anglickém jazyce

miRNA sequencing shows different gene expression in adult and infantile epilepsy

Popis výsledku anglicky

Epilepsy is a severe neurological disorder characterized by recurrent seizures. The onset of seizure activity frequently occurs in infancy and early childhood. During the early life period, the neuronal networks are not fully functional, and novel neuronal connections are rapidly formed. Hence, the seizures might have different impact on the adult and developing brain. It was previously shown that the rats with epilepsy onset in the infancy suffer from less severe brain damage later in life than the rats with epilepsy onset in the adulthood. Altered microRNA (short noncoding RNA; miRNA) profile has been associated with epilepsy in numerous studies dealing with both epileptic patients and animal models. To address the differences in gene expression regulation in epilepsy with onset in infancy and adulthood, we performed the analysis of miRNA profiles in infant (12 days old) and adult rats. miRNA profiles were analyzed in the hippocampus (epilepsy onset zone) of male Wistar rats with chemically induced status epilepticus (SE). Tissue samples were collected at two timepoints: 24 hours (acute phase) and 3 months after SE (chronic phase with spontaneous seizures). Sequencing analysis showed that the dysregulation of multiple miRNAs was concordant in both infantile and adult rats in acute phase. Interestingly, numerous miRNAs which were dysregulated in acute phase showed the similar trend in chronic phase in rats with SE in adulthood but not in rats with SE during infancy. These results show that the epilepsy onset in infancy and adulthood alters the regulation of gene expression which might indicate different pathological processes and necessary adjustment of treatment.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30100 - Basic medicine

Projekt

<a href="/cs/project/GA16-04726S" target="_blank" >GA16-04726S: miRNA a biochemické dráhy v epilepsii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů