Dynamics of microRNA expression during early onset epileptogenesis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F18%3A00101482" target="_blank" >RIV/00216224:14740/18:00101482 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1111/epi.14611" target="_blank" >http://dx.doi.org/10.1111/epi.14611</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/epi.14611" target="_blank" >10.1111/epi.14611</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dynamics of microRNA expression during early onset epileptogenesis

Popis výsledku v původním jazyce

Purpose: Seizures and epilepsy have been associated with altered microRNA (miRNA/miR) profile in the brain. Epilepsy frequently starts in early childhood when brain is immature and neuronal networks are not fully functional. Since changes in miRNA expression might influence pathways essential for brain development, deciphering miRNA dynamics of early onset epilepsies might provide insight into mechanism underlying seizure related alterations in the developing brain. The aim of this study was to identify changes in miRNA profile during the epileptogenesis triggered by status epilepticus (SE) in infant rats (P12). Method: Rats were exposed to LiCl/pilocarpine induced SE at postnatal (P) day 12 (n=10). Controls (n=10) were treated the same way except they received saline instead of pilocarpine. Hippocampal tissues were collected at three timepoints: 24 hours (acute), 7days (latent) and 3 months (chronic stage) after SE. Total RNA was isolated and small RNA sequencing was used to address miRNA profiles in all tissue specimens. Results: Sequencing analysis showed that all analyzed stages of epileptogenesis had unique hippocampal miRNA profiles. Majority of miRNAs had altered expression only at specific timepoint,whilst expression levels of miR-155-5p; -22-3p and -24-3p was elevated in both acute and chronic stage and miR-21-5p and 24-2-5p was upregulated in animals after SE in all stages. Conclusion: Epilepsy development in the immature brain leads to changes in miRNA profile that might severely impact the gene expression. The effect of seizures on the brain development might be triggered by elevated expression of miRNAs specific for acute and chronic epilepsy stages characterized by seizures and epileptic comorbidities.

Název v anglickém jazyce

Dynamics of microRNA expression during early onset epileptogenesis

Popis výsledku anglicky

Purpose: Seizures and epilepsy have been associated with altered microRNA (miRNA/miR) profile in the brain. Epilepsy frequently starts in early childhood when brain is immature and neuronal networks are not fully functional. Since changes in miRNA expression might influence pathways essential for brain development, deciphering miRNA dynamics of early onset epilepsies might provide insight into mechanism underlying seizure related alterations in the developing brain. The aim of this study was to identify changes in miRNA profile during the epileptogenesis triggered by status epilepticus (SE) in infant rats (P12). Method: Rats were exposed to LiCl/pilocarpine induced SE at postnatal (P) day 12 (n=10). Controls (n=10) were treated the same way except they received saline instead of pilocarpine. Hippocampal tissues were collected at three timepoints: 24 hours (acute), 7days (latent) and 3 months (chronic stage) after SE. Total RNA was isolated and small RNA sequencing was used to address miRNA profiles in all tissue specimens. Results: Sequencing analysis showed that all analyzed stages of epileptogenesis had unique hippocampal miRNA profiles. Majority of miRNAs had altered expression only at specific timepoint,whilst expression levels of miR-155-5p; -22-3p and -24-3p was elevated in both acute and chronic stage and miR-21-5p and 24-2-5p was upregulated in animals after SE in all stages. Conclusion: Epilepsy development in the immature brain leads to changes in miRNA profile that might severely impact the gene expression. The effect of seizures on the brain development might be triggered by elevated expression of miRNAs specific for acute and chronic epilepsy stages characterized by seizures and epileptic comorbidities.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů