Formation of Staphylococcus aureus biofilm in the presence of sub-lethal concentrations of disinfectants: a transcriptomic analysis using RNA-seq

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F17%3A00098003" target="_blank" >RIV/00216224:14740/17:00098003 - isvavai.cz</a>

Výsledek na webu

<a href="http://aem.asm.org/content/early/2017/10/09/AEM.01643-17" target="_blank" >http://aem.asm.org/content/early/2017/10/09/AEM.01643-17</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/AEM.01643-17" target="_blank" >10.1128/AEM.01643-17</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Formation of Staphylococcus aureus biofilm in the presence of sub-lethal concentrations of disinfectants: a transcriptomic analysis using RNA-seq

Popis výsledku v původním jazyce

Staphylococcus aureus is a common biofilm-forming pathogen. Low doses of disinfectants have previously been reported to promote biofilm formation and to increase virulence. The aim of this study was to use RNA-seq analysis to investigate global transcriptional changes in S. aureus in response to sub-lethal concentrations of commonly used food industry disinfectants (ethanol, Et; chloramine T, ChT; and their combination, Et_ChT), in order to better understand the effects of these agents on biofilm formation. Treatment with Et and Et_ChT resulted in more significantly altered expression profiles, compared to ChT. Our results revealed that Et and Et_ChT treatments enhanced the expression of genes responsible for regulation of gene expression (sigB), cell surface factors (clfAB), adhesins (sdrDE) and capsular polysaccharides (cap8EFGL) resulting in more intact biofilm. In addition, in this study we were able to identify the pathways involved in the adaptation of S. aureus to the stress of ChT treatment. Further, Et suppressed the effect of ChT on gene expression when these agents were used together at sub-lethal concentrations. These data show that in the presence of sub-lethal concentrations of tested disinfectants, S. aureus cells triggers protective mechanisms and tries to cope with them.

Název v anglickém jazyce

Formation of Staphylococcus aureus biofilm in the presence of sub-lethal concentrations of disinfectants: a transcriptomic analysis using RNA-seq

Popis výsledku anglicky

Staphylococcus aureus is a common biofilm-forming pathogen. Low doses of disinfectants have previously been reported to promote biofilm formation and to increase virulence. The aim of this study was to use RNA-seq analysis to investigate global transcriptional changes in S. aureus in response to sub-lethal concentrations of commonly used food industry disinfectants (ethanol, Et; chloramine T, ChT; and their combination, Et_ChT), in order to better understand the effects of these agents on biofilm formation. Treatment with Et and Et_ChT resulted in more significantly altered expression profiles, compared to ChT. Our results revealed that Et and Et_ChT treatments enhanced the expression of genes responsible for regulation of gene expression (sigB), cell surface factors (clfAB), adhesins (sdrDE) and capsular polysaccharides (cap8EFGL) resulting in more intact biofilm. In addition, in this study we were able to identify the pathways involved in the adaptation of S. aureus to the stress of ChT treatment. Further, Et suppressed the effect of ChT on gene expression when these agents were used together at sub-lethal concentrations. These data show that in the presence of sub-lethal concentrations of tested disinfectants, S. aureus cells triggers protective mechanisms and tries to cope with them.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/LM2015043" target="_blank" >LM2015043: Česká infrastruktura pro integrativní strukturní biologii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

APPLIED AND ENVIRONMENTAL MICROBIOLOGY

ISSN

0099-2240

e-ISSN

—

Svazek periodika

83

Číslo periodika v rámci svazku

24

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

13

Strana od-do

—

Kód UT WoS článku

000416937000008

EID výsledku v databázi Scopus

2-s2.0-85036469590